Biotin ELISA Kit
Biotin ELISA Kit from ELK Biotechnology Read more ›
Product Description
Biotin ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of Biotin.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Biotin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Biotin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Biotin in the samples is then determined by comparing the OD of the samples to the standard curve.
Target background
Alternative names include Biotin.
Key Facts
| Target | Biotin |
| Also known as | Biotin |
| Species reactivity | General |
| Form / buffer | Pre-coated microplate kit |
| Research area | Enzyme & Kinase |
| Assay type | Competitive Inhibition |
| Detection method | Colorimetric (TMB/HRP) |
| Sensitivity | 4.7 pg/mL |
| Detection range | 15.63-1000 pg/mL |
| Sample types |
SerumPlasmaCell lysatesCell culture supernatantsOther biological fluids
See sample validation ›
|
| Plate format | 96-well pre-coated |
Sample Type Validation
1 sample type| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| Sample compatibility |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.