Rabbit C5a(Complement Component 5a) ELISA Kit
Rabbit C5a(Complement Component 5a) ELISA Kit from ELK Biotechnology Read more ›
Product Description
Rabbit C5a(Complement Component 5a) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of C5a.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit C5a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit C5a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit C5a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit C5a in the samples is then determined by comparing the OD of the samples to the standard curve.
Target background
Alternative names include Complement Component 5a.
Key Facts
| Target | C5a |
| Also known as | Complement Component 5a |
| Species reactivity | Rabbit |
| Form / buffer | Pre-coated microplate kit |
| Research area | Infection immunity, Immune molecule, Autoimmunity |
| Assay type | Sandwich |
| Detection method | Colorimetric (TMB/HRP) |
| Sensitivity | 0.57 ng/mL |
| Detection range | 1.57-100 ng/mL |
| Sample types |
SerumPlasmaOther biological fluids
See sample validation ›
|
| Plate format | 96-well pre-coated |
Sample Type Validation
1 sample type| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| Sample compatibility |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.