Rat S100A11(S100 Calcium Binding Protein A11) ELISA Kit

Rat S100A11(S100 Calcium Binding Protein A11) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of S100A11.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat S100A11. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat S100A11. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat S100A11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat S100A11 in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include S100-A11; S100C; MLN70; Protein S100-C; Calgizzarin; Metastatic lymph node gene 70 protein; Protein S100-A11, N-terminally processed.