Mouse 15d-PGJ2 (15-Deoxy-delta 12,14-prostaglandin J2) ELISA Kit
Mouse 15d-PGJ2 (15-Deoxy-delta 12,14-prostaglandin J2) ELISA Kit from ELK Biotechnology Read more ›
Product Description
Mouse 15d-PGJ2 (15-Deoxy-delta 12,14-prostaglandin J2) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of 15d-PGJ2.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse 15d-PGJ2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse 15d-PGJ2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse 15d-PGJ2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Target background
Alternative names include 15-Deoxy-delta 12,14-prostaglandin J2.
Key Facts
| Target | 15d-PGJ2 |
| Also known as | 15-Deoxy-delta 12,14-prostaglandin J2 |
| Species reactivity | Mouse |
| Form / buffer | Pre-coated microplate kit |
| Research area | Endocrinology, Pulmonology, Hepatology, Hormone metabolism |
| Assay type | Competitive Inhibition |
| Detection method | Colorimetric (TMB/HRP) |
| Sensitivity | 1.23 ng/mL |
| Detection range | 3.13-200 ng/mL |
| Sample types |
SerumPlasmaOther biological fluids
See sample validation ›
|
| Plate format | 96-well pre-coated |
Sample Type Validation
1 sample type| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| Sample compatibility |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.