Human 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit
Human 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit from ELK Biotechnology Read more ›
Product Description
Human 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of 25-OH-D.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 25-OH-D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 25-OH-D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.
Target background
Alternative names include 25 Hydroxy Vitamin D;.
Key Facts
| Target | 25-OH-D |
| Also known as | 25 Hydroxy Vitamin D; |
| Species reactivity | Human |
| Form / buffer | Pre-coated microplate kit |
| Research area | Reproductive science, Genetic science, Nutrition metabolism, Bone metabolism |
| Assay type | Competitive Inhibition |
| Detection method | Colorimetric (TMB/HRP) |
| Sensitivity | 1.45 ng/mL |
| Detection range | 3.13-200 ng/mL |
| Sample types |
SerumPlasmaOther biological fluids
See sample validation ›
|
| Plate format | 96-well pre-coated |
Sample Type Validation
1 sample type| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| Sample compatibility |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.