EasyStep Human IGFBP3(Insulin Like Growth Factor Binding Protein 3) ELISA Kit

EasyStep Human IGFBP3(Insulin Like Growth Factor Binding Protein 3) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of EasyStep Human IGFBP3.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP3, and the Human IGFBP3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IGFBP3. After TMB substrate solution is added, only those wells that contain Human IGFBP3 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IGFBP3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include BP53; IBP3; Growth Hormone-Dependent Binding Protein; Acid Stable Subunit Of The 140 K IGF Complex.