EasyStep Rat fT3(Free Triiodothyronine) ELISA Kit
EasyStep Rat fT3(Free Triiodothyronine) ELISA Kit from ELK Biotechnology Read more ›
Product Description
EasyStep Rat fT3(Free Triiodothyronine) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of EasyStep Rat fT3.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat fT3 antigen, and the Rat fT3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat fT3 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat fT3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Target background
Alternative names include 3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel; Tertroxin.
Key Facts
| Target | EasyStep Rat fT3 |
| Also known as | 3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel; Tertroxin |
| Species reactivity | Rat |
| Form / buffer | Pre-coated microplate kit |
| Research area | Endocrinology, Autoimmunity |
| Assay type | Competitive Inhibition |
| Detection method | Colorimetric (TMB/HRP) |
| Sensitivity | 0.31 ng/mL |
| Detection range | 0.32-20 ng/mL |
| Sample types |
SerumPlasma
See sample validation ›
|
| Plate format | 96-well pre-coated |
Sample Type Validation
1 sample type| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| Sample compatibility |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.