Human NKX2-5(NK2 Homeobox 5) ELISA Kit

Human NKX2-5(NK2 Homeobox 5) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of NKX2-5.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NKX2-5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NKX2-5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NKX2-5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NKX2-5 in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include Cardiac-specific homeobox; Homeobox protein CSX; Homeobox protein NK-2 homolog E; CSX; NKX2.5; NKX2E.