Human SSC5D(Soluble scavenger receptor cysteine-rich domain-containing protein SSC5D) ELISA Kit

Human SSC5D(Soluble scavenger receptor cysteine-rich domain-containing protein SSC5D) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of SSC5D.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SSC5D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SSC5D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SSC5D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SSC5D in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include SRCRL; Soluble scavenger protein with 5 SRCR domains; SSc5D.