Horse 5-HT(5-Hydroxytryptamine) ELISA Kit
Horse 5-HT(5-Hydroxytryptamine) ELISA Kit from ELK Biotechnology Read more ›
Product Description
Horse 5-HT(5-Hydroxytryptamine) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of Horse 5-HT.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Horse 5-HT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.
Target background
Alternative names include 5HT; Serotonin; N-Methyl-Gamma.
Key Facts
| Target | Horse 5-HT |
| Also known as | 5HT; Serotonin; N-Methyl-Gamma |
| Species reactivity | Horse |
| Form / buffer | Pre-coated microplate kit |
| Research area | Signal transduction, Infection immunity, Neuro science, Gastroenterology |
| Assay type | Competitive Inhibition |
| Detection method | Colorimetric (TMB/HRP) |
| Sensitivity | 5.85 ng/mL |
| Detection range | 14.07-900 ng/mL |
| Sample types |
SerumPlasma
See sample validation ›
|
| Plate format | 96-well pre-coated |
Sample Type Validation
1 sample type| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| Sample compatibility |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.