Human MuRF1(muscle-specific RING-finger protein 1) ELISA Kit

Human MuRF1(muscle-specific RING-finger protein 1) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of MuRF1.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MuRF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MuRF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MuRF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MuRF1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include MuRF1; IRF; RNF28; SMRZ; TRIM63; IRFMURF2; Iris RING finger protein;MURF1; MuRF-1; muscle specific ring finger protein 2; Muscle-specific RING finger protein 1; RING finger protein 28FLJ32380; RNF28E3 ubiquitin-protein ligase TRIM63; SMRZMURF-1; Striated muscle RING zinc finger protein; tripartite motif containing 63;.