EasyStep Rat E2(Estradiol) ELISA Kit
EasyStep Rat E2(Estradiol) ELISA Kit from ELK Biotechnology Read more ›
Product Description
EasyStep Rat E2(Estradiol) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of EasyStep Rat E2.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat E2 antigen, and the Rat E2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat E2 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat E2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Target background
Alternative names include 17B-Estradiol; Oestradiol; Beta-Estradiol.
Key Facts
| Target | EasyStep Rat E2 |
| Also known as | 17B-Estradiol; Oestradiol; Beta-Estradiol |
| Species reactivity | Rat |
| Form / buffer | Pre-coated microplate kit |
| Research area | Endocrinology, Reproductive science, Hormone metabolism |
| Assay type | Competitive Inhibition |
| Detection method | Colorimetric (TMB/HRP) |
| Sensitivity | 25 pg/mL |
| Detection range | 25-1600 pg/mL |
| Sample types |
Serum
See sample validation ›
|
| Plate format | 96-well pre-coated |
Sample Type Validation
1 sample type| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| Sample compatibility |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.