8-iso-PGF2α(8-isoprostane) ELISA Kit
8-iso-PGF2α(8-isoprostane) ELISA Kit from ELK Biotechnology Read more ›
Product Description
8-iso-PGF2α(8-isoprostane) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of 8-iso-PGF2α.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-iso-PGF2α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-iso-PGF2α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-iso-PGF2α in the samples is then determined by comparing the OD of the samples to the standard curve.
Target background
Alternative names include 8-isoprostane; 8-iso-PGF2α.
Key Facts
| Target | 8-iso-PGF2α |
| Also known as | 8-isoprostane; 8-iso-PGF2α |
| Species reactivity | General |
| Form / buffer | Pre-coated microplate kit |
| Research area | Endocrinology, Pulmonology, Hepatology, Hormone metabolism |
| Assay type | Competitive Inhibition |
| Detection method | Colorimetric (TMB/HRP) |
| Sensitivity | 4.47 pg/mL |
| Detection range | 15.63-1000 pg/mL |
| Sample types |
SerumPlasmaOther biological fluids
See sample validation ›
|
| Plate format | 96-well pre-coated |
Sample Type Validation
1 sample type| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| Sample compatibility |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.