Rat SREBP-1C(Sterol Regulatory Element-Binding Protein 1C) ELISA Kit
Rat SREBP-1C(Sterol Regulatory Element-Binding Protein 1C) ELISA Kit from ELK Biotechnology Read more ›
Product Description
Rat SREBP-1C(Sterol Regulatory Element-Binding Protein 1C) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of SREBP-1C.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SREBP-1C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SREBP-1C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SREBP-1C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SREBP-1C in the samples is then determined by comparing the OD of the samples to the standard curve.
Target background
Alternative names include ADD-1; ADD1; SREBP-1; SREBP-1c; Srebp1.
Key Facts
| Target | SREBP-1C |
| Also known as | ADD-1; ADD1; SREBP-1; SREBP-1c; Srebp1 |
| Species reactivity | Rat |
| Form / buffer | Pre-coated microplate kit |
| Research area | Cardiovascular, Epigenetics and Nuclear Signaling, Cancer, Metabolism |
| Assay type | Sandwich |
| Detection method | Colorimetric (TMB/HRP) |
| Sensitivity | 18.3 pg/mL |
| Detection range | 62.5-4000 pg/mL |
| Sample types |
SerumPlasmaTissue homogenatesCell lysatesCell culture supernatantsOther biological fluids
See sample validation ›
|
| Plate format | 96-well pre-coated |
Sample Type Validation
1 sample type| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT | |
|---|---|---|---|---|---|---|
| Sample compatibility |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.