ABM92195
P53 Rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
P53 Rabbit pAb from ELK Biotechnology Read more ›
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Product Description
P53 Rabbit pAb is an ELK Biotechnology antibody for research workflows involving P53.
Background
p53 plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro.
Additional antibody information
| Validation evidence captions | Western blot analysis of 1) Mouse Brain Tissue, 2)Rat Brain Tissue with p53 Rabbit pAb EA173 diluted at 1:2,000. | Immunohistochemical analysis of paraffin-embedded Mouse Colon Tissue using p53（EA173）Rabbit pAb diluted at 1:500. |
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Key Facts
Product type
Primary Antibody›
Target: P53
| Target | P53 |
| Also known as | Cellular tumor antigen p53 antibody, Cys 51 stop antibody, Tp53 antibody, Tumor protein p53 antibody |
| Concentration | 1mg/mL |
| Working concentration | WB 1:1,000-2,000 IHC 1:500-1,000 |
| Species reactivity | Rat, Mouse |
| Observed band | 53kDa |
| Cellular localization | Cytoplasm, Cytoplasm, Nucleus |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:1,000-2,000 IHC 1:500-1,000 |
| Immunogen | Synthetic Peptide |
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Citations
ISO 9001
Certified Collaborators
Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Rat | WB 1:1 | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
Specifications
Target
P53
Also known as
Cellular tumor antigen p53 antibody, Cys 51 stop antibody, Tp53 antibody, Tumor protein p53 antibody
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000 IHC 1:500-1,000
Form / buffer
Antibody solution
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Rat, Mouse
Tested applications
WBIHC
Immunogen
Synthetic Peptide
Observed band
53kDa
Cellular localization
Cytoplasm, Cytoplasm, Nucleus
Dilution range
WB 1:1,000-2,000 IHC 1:500-1,000
Storage & Stability
Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Storage conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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