ABM92165
Histone H3(Phospho Thr32 ) Rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
Histone H3(Phospho Thr32 ) Rabbit pAb from ELK Biotechnology Read more ›
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Product Description
Histone H3(Phospho Thr32 ) Rabbit pAb is an ELK Biotechnology antibody for research workflows involving Histone H3(Phospho Thr32 ).
Background
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Additional antibody information
| Validation evidence captions | Western blot analysis of extracts from Hela cells, untreated (-) or treated with Nocodazole (1µg/ml, 24 hr; +), using Phospho-Histone H3 (Thr32) Rabbit pAb (upper) EA064 1:2000 and Histone H3 (1G1) Mouse mAb (lower) EM1037 1:2000. | Immunohistochemical analysis of paraffin-embedded Human Breast Caricnoma using Histone H3(Phospho Thr32)（EA064）Rabbit pAb diluted at 1:500. |
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Key Facts
Product type
Primary Antibody›
Target: Histone H3(Phospho Thr32 )
| Target | Histone H3(Phospho Thr32 ) |
| Also known as | H3 histone antibody, HIST1H3A antibody, Histone cluster 1, H7a antibody |
| Concentration | 1mg/mL |
| Working concentration | WB 1:1,000-2,000 IHC 1:200-500 |
| Species reactivity | Human, Rat, Mouse |
| Observed band | 15-17kDa |
| Cellular localization | Nuclear |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:1,000-2,000 IHC 1:200-500 |
| Immunogen | Synthetic Peptide |
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Citations
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1 | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
Specifications
Target
Histone H3(Phospho Thr32 )
Also known as
H3 histone antibody, HIST1H3A antibody, Histone cluster 1, H7a antibody
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000 IHC 1:200-500
Form / buffer
Antibody solution
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human, Rat, Mouse
Tested applications
WBIHC
Immunogen
Synthetic Peptide
Observed band
15-17kDa
Cellular localization
Nuclear
Dilution range
WB 1:1,000-2,000 IHC 1:200-500
Storage & Stability
Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Storage conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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