Flag-Tag Rabbit pAb
Flag-Tag Rabbit pAb from ELK Biotechnology Read more ›
Product Description
Flag-Tag Rabbit pAb is an ELK Biotechnology antibody for research workflows involving Flag-Tag.
Background
The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.
Additional antibody information
| Validation evidence captions | 1ug Flag fusion protein+ Primary antibody dilution at 1、1:5,000 2、1:10,000 | Transfected 293 cell lysate with Flag-tag fusion protein+ Primary antibody dilution at 1:10,000 |
|---|
Key Facts
| Target | Flag-Tag |
| Concentration | 1mg/mL |
| Working concentration | WB 1:5,000 |
| Species reactivity | Epitope tag |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB 1:5,000 |
| Immunogen | Synthetic Peptide |
Reactivity & Application Validation
1 target type| Target type | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Epitope tag |
| Target type | Dilution | Notes |
|---|---|---|
| Epitope tag | WB 1:5 | — |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.