Home » Calibrated High Molecular Weight Color Prestained Protein Marker (25–400 kDa)
Protein Marker
ABM55

Calibrated High Molecular Weight Color Prestained Protein Marker (25–400 kDa)

Ready-to-UseColorful BandsIdeal for Macromolecular Proteins

A high-purity large molecule recombinant colour prestained protein marker specifically designed for research experiments. Through scientific proportioning of 10 precise molecular weight proteins (25, 45, 72, 100, 130,... Read more ›

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Calibrated High Molecular Weight Color Prestained Protein Marker (25–400 kDa) - ABMIUM
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Calibrated High Molecular Weight Color Prestained Protein Marker (25–400 kDa) - ABMIUM
Calibrated High Molecular Weight Color Prestained Protein Marker (25–400 kDa)

Product Description

A high-purity large molecule recombinant colour prestained protein marker specifically designed for research experiments. Through scientific proportioning of 10 precise molecular weight proteins (25, 45, 72, 100, 130, 160, 200, 250, 300, 400kDa), it addresses the core pain points of traditional prestained markers "low accuracy and poor stability" providing key support for improved experimental efficiency.
Color differentiation design: 72kDa (orange-red), 25kDa (green), others (blue), allowing real-time observation without staining, no need to pause electrophoresis to judge progress, quick confirmation of transfer efficiency after membrane transfer
Authoritative calibration guarantee: Calibrated with imported brand non-prestained marker standards, molecular weight deviation is far below industry average, accuracy comparable to non-prestained markers
  • Pre-mixed 1× SDS-PAGE loading buffer, no boiling required
  • Specialized for large molecules: 25-400kDa suitable for most research large molecule proteins
  • Clear band performance: 5-10μL loading volume produces sharp bands, no diffusion or trailing after electrophoresis
Product Name Specification 1 (50μL) Specification 2 (250μL)
Calibrated High Molecular Weight Color Prestained Protein Marker (25-400kDa) Suitable for small batchpre-experiments (approx. 5-10 uses) Suitable for regular laboratories (approx. 25-50 uses)

 

1. High Molecular Weight Accuracy: Comprehensive Leadership Over Domestic and International Counterparts

This product's accuracy significantly surpasses international brands; while deviation at 72kDa is relatively large, all other bands perform equally or better than international brands.

 

2. Buffer System Compatibility: Stable Migration Across Scenarios

Validated across multiple systems including Tris-Glycine buffer (conventional SDS-PAGE system), Tris-Tricine buffer (small molecule protein separation system), and Bis-Tris buffer (high-resolution separation system), this product maintains stable migration in all systems

 

3. Extreme Condition Stability: Handling Complex Experimental Environments

Repeated freeze-thaw testing: 50 freeze-thaw cycles (-20℃→room temperature), no significant changes in band clarity or molecular weight migration, meeting laboratory scenarios requiring "frequent access"
High temperature stability testing: 7 days at 40℃ simulating transportation environment, no protein degradation or aggregation, band integrity maintained well, addressing "summer transportation deterioration" concernsRoom temperature storage testing: 30 days at 25℃ room temperature, no observed band changes, short-term forgetting to return to refrigerator does not affect use


4. Operational Convenience: Cost-Reducing, Efficiency-Enhancing Research Assistant

  • No boiling required: Avoids traditional marker "boiling denaturation" step, reduces protein loss, and lowers operational risks
  • No dilution required: Pre-mixed 1× loading buffer, ready for direct loading, easy for beginners to use quickly

 

Instructions for Use

  1. Remove product from -20℃ freezer, place in 4℃ refrigerator (10-20 minutes) or room temperature (5-8 minutes) for natural thawing, strictly avoid water bath heating or boiling;
  2. After thawing, gently mix (recommend centrifugation at 4000-8000rpm for 30s before mixing);
  3. Aspirate 5-10μL, slowly add to gel loading well, adjust loading volume appropriately according to gel properties and experimental habits;
  4. Return unused marker to -20℃, recommend aliquoting into 20-50μL/tube.

 

Precautions

  • This product already contains loading buffer, do not heat or boil;
  • To reduce marker degradation and contamination risks, recommend aliquoting and storing at -20℃;
  • For research use only.

Key Facts

Product type Protein Marker
Shelf life 2 years from manufacture
Why researchers trust ABMIUM
2,000+ Researchers trust us
50,000+ Citations
ISO 9001 Certified Collaborators

Specifications

Storage & Stability

Shipped at
Ice bag
Short-term storage
Storage at 4°C is valid for 2 months.
Long-term storage
Storage at -20°C is valid for 2 years
Shelf life
2 years from manufacture

Compliance & Certifications

ISO 9001:2015
ISO 9001:2015 Certified

Manufactured under ISO 9001:2015 quality management standards.

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

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