Primary Antibody ABMIUM Verified™Collaborator Tested
ABM-34082

Anti-Zebrafish HMGB1a/b Antibody

ABMIUM Verified™|Collaborator Tested|Boster Bio

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Anti-Zebrafish HMGB1a/b Antibody Boster Bio
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Anti-Zebrafish HMGB1a/b Antibody Boster Bio

Product Description

Boster Bio Anti-Zebrafish-HMGB1a-b-Antibody catalog # AZQ6NX86. Tested in WB, IHC applications. This antibody reacts with Zebrafish.

High mobility group box 1 protein, also known as high-mobility group protein 1 (HMG-1) and amphoterin, is a protein that in humans is encoded by the HMGB1 gene. This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.

Product information

Supplier Boster Bio
ABMIUM evidence status ABMIUM Verified™ collaborator-tested data
Catalogue reference AZQ6NX86
Product category Primary Antibodies, Zebrafish Antibodies
Reactive species Zebrafish
Tested applications IHC, IHC-P, WB
Available conjugations Unconjugated, Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, Fluoro647
Gene name HMGB1a/b
Protein name High mobility group box 1a
Immunogen E.coli-derived zebrafish HMGB1a/b recombinant protein (Position: D123-K153).
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Purification Immunogen affinity purified.
Storage At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
UniProt Q6NX86
NCBI Gene ID 321622

Available variants

Conjugation Size
Unconjugated 100 μg/vial
Biotin 100 μg/vial
Cy3 100 μg/vial
Fluoro488 100 μg/vial
Fluoro550 100 μg/vial
Fluoro594 100 μg/vial
FITC 100 μg/vial
HRP 100 μg/vial
APC 100 μg/vial
PE 100 μg/vial
Fluoro647 100 μg/vial

Key Facts

Product type Primary Antibody Target: High mobility group box 1a
Target High mobility group box 1a
Also known as high mobility group box 1; High mobility group protein 1; High mobility group protein B1; HMG 1; HMG1; HMG3; HMGB1; SBP 1
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Species reactivity Zebrafish
Form / buffer Lyophilized
Shelf life At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host species Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Conjugation Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Tested applications
IHCIHC-PWB
Dilution range Western blot, 0.25-0.5 μg/ml, Zebrafish
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Zebrafish
Immunogen E.coli-derived zebrafish HMGB1a/b recombinant protein (Position: D123-K153).
Purification Immunogen affinity purified.
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Reactivity & Application Validation

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFLOW CYTOMETRYELISAIP
Zebrafish

Specifications

Target
High mobility group box 1a
Also known as
high mobility group box 1; High mobility group protein 1; High mobility group protein B1; HMG 1; HMG1; HMG3; HMGB1; SBP 1
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form / buffer
Lyophilized
Host species
Rabbit
Clonality
Polyclonal
Isotype
Rabbit IgG
Conjugation
Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Species reactivity
Zebrafish
Tested applications
IHCIHC-PWB
Immunogen
E.coli-derived zebrafish HMGB1a/b recombinant protein (Position: D123-K153).
Dilution range
Western blot, 0.25-0.5 μg/ml, Zebrafish
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Zebrafish
Purification
Immunogen affinity purified.

Storage & Stability

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Long-term storage
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Storage conditions
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Storage buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Shelf life
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Compliance & Certifications

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

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