ABM-29332
Anti-ZAP70 Monoclonal Antibody
ABMIUM Verified™|Collaborator Tested|Boster Bio
Primary Antibody supplied by Boster Bio Read more ›
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Product Description
Boster Bio Anti-ZAP70 Monoclonal Antibody catalog # M00754-3. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | M00754-3 |
| Product category | Primary Antibodies, Rabbit Monoclonal Antibodies |
| Reactive species | Human |
| Tested applications | Flow Cytometry, IF/ICC, IHC, IP, WB |
| Available conjugations | Unconjugated |
| Gene name | ZAP70 |
| Protein name | Tyrosine-protein kinase ZAP-70 |
| Immunogen | A synthesized peptide derived from human ZAP70 The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement. Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck. |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Purification | Affinity-chromatography |
| Storage | Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles. |
| UniProt | P43403 |
| NCBI Gene ID | 7535 |
Available variants
| Conjugation | Size |
|---|---|
| Unconjugated | 100 μl/vial |
Key Facts
Product type
Primary Antibody›
Target: Tyrosine-protein kinase ZAP-70
| Target | Tyrosine-protein kinase ZAP-70 |
| Also known as | ADMIO2; IMD48; SRK; STCD; STD; TZK; ZAP-70; ZAP70; ZAP 70 |
| Concentration | 0.5mg/ml |
| Species reactivity | Human |
| Observed band | 100 kDa |
| Form / buffer | Liquid |
| Shelf life | Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles. |
| Host species | Rabbit |
| Clonality | Monoclonal |
| Clone number | Clone: ACAF-26 |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Tested applications |
Flow CytometryIF/ICCIHCIPWB |
| Dilution range | WB 1:500-2000 IHC 1:50-200 ICC/IF 1:50-200 IP 1:20 FC 1:20 |
| Immunogen | A synthesized peptide derived from human ZAP70 The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement. Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck. |
| Purification | Affinity-chromatography |
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Reactivity & Application Validation
1 species| Species | WB | IHC | IHC-P | IHC-F | IF/ICC | FLOW CYTOMETRY | ELISA | IP |
|---|---|---|---|---|---|---|---|---|
| Human |
Specifications
Target
Tyrosine-protein kinase ZAP-70
Also known as
ADMIO2; IMD48; SRK; STCD; STD; TZK; ZAP-70; ZAP70; ZAP 70
Concentration
0.5mg/ml
Form / buffer
Liquid
Host species
Rabbit
Clonality
Monoclonal
Clone number
Clone: ACAF-26
Isotype
Rabbit IgG
Conjugation
Unconjugated
Species reactivity
Human
Tested applications
Flow CytometryIF/ICCIHCIPWB
Immunogen
A synthesized peptide derived from human ZAP70 The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement. Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck.
Observed band
100 kDa
Dilution range
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
Purification
Affinity-chromatography
Storage & Stability
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Long-term storage
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Storage conditions
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Storage buffer
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Shelf life
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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