Primary Antibody ABMIUM Verified™Collaborator Tested
ABM-26333

Anti-Smad9 Antibody Picoband®

ABMIUM Verified™|Collaborator Tested|Boster Bio

Primary Antibody supplied by Boster Bio Read more ›

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Anti-Smad9 Antibody Picoband® Boster Bio
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Anti-Smad9 Antibody Picoband® Boster Bio

Product Description

Boster Bio Anti-Smad9 Antibody Picoband® catalog # A07303-1. Tested in WB, ELISA applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Mothers against decapentaplegic homolog 9 also known as SMAD9, SMAD8, and MADH6 is a protein that in humans is encoded by the SMAD9 gene. This gene encodes a member of a family of proteins that act as downstream effectors of the bone morphogenetic protein (BMP) signaling pathway. The encoded protein is phosphorylated by BMP receptors, which stimulates its binding to SMAD4 and translocation into the nucleus, where it functions as a regulator of transcription. Activity of this protein is important for embryonic development. Mutation of this gene results in defects in pulmonary vasculature.

Product information

Supplier Boster Bio
ABMIUM evidence status ABMIUM Verified™ collaborator-tested data
Catalogue reference A04932-1
Product category Primary Antibodies
Reactive species Mouse, Rat
Tested applications ELISA, Flow Cytometry, IHC, IHC-P, WB
Available conjugations Unconjugated, Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, Fluoro647
Gene name Smad9
Protein name Mothers against decapentaplegic homolog 9
Immunogen E.coli-derived mouse Smad9 recombinant protein (Position: Q153-H235). Mouse Smad9 shares 59.2% and 96.4% amino acid (aa) sequence identity with human and rat Smad9, respectively.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Purification Immunogen affinity purified.
Storage At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
UniProt Q9JIW5
NCBI Gene ID 55994

Available variants

Conjugation Size
Unconjugated 100 μg/vial
Biotin 100 μg/vial
Cy3 100 μg/vial
Fluoro488 100 μg/vial
Fluoro550 100 μg/vial
Fluoro594 100 μg/vial
FITC 100 μg/vial
HRP 100 μg/vial
APC 100 μg/vial
PE 100 μg/vial
Fluoro647 100 μg/vial

Key Facts

Product type Primary Antibody Target: Mothers against decapentaplegic homolog 9
Target Mothers against decapentaplegic homolog 9
Also known as MAD homolog 9; MADH6; MADH9; Mothers against DPP homolog 9; SMAD 9; SMAD family member 9; SMAD8A; SMAD8B; SMAD9
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Species reactivity Mouse,Rat
Observed band 55 kDa
Form / buffer Lyophilized
Shelf life At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
Host species Rabbit
Clonality Polyclonal
Isotype IgG
Conjugation Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Non-cross-reactive with No cross reactivity with other proteins.
Tested applications
ELISAFlow CytometryIHCIHC-PWB
Dilution range Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Immunogen E.coli-derived mouse Smad9 recombinant protein (Position: Q153-H235). Mouse Smad9 shares 59.2% and 96.4% amino acid (aa) sequence identity with human and rat Smad9, respectively.
Purification Immunogen affinity purified.
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Reactivity & Application Validation

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFLOW CYTOMETRYELISAIP
Mouse
Rat

Specifications

Target
Mothers against decapentaplegic homolog 9
Also known as
MAD homolog 9; MADH6; MADH9; Mothers against DPP homolog 9; SMAD 9; SMAD family member 9; SMAD8A; SMAD8B; SMAD9
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form / buffer
Lyophilized
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Species reactivity
Mouse,Rat
Tested applications
ELISAFlow CytometryIHCIHC-PWB
Immunogen
E.coli-derived mouse Smad9 recombinant protein (Position: Q153-H235). Mouse Smad9 shares 59.2% and 96.4% amino acid (aa) sequence identity with human and rat Smad9, respectively.
Observed band
55 kDa
Dilution range
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Purification
Immunogen affinity purified.

Storage & Stability

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
Long-term storage
At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
Storage conditions
At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
Storage buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Shelf life
At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Compliance & Certifications

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

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