Anti-Nitric Oxide Synthase, Brain(1-181) NOS1 Antibody (Monoclonal, NOS-B1)
Primary Antibody supplied by Boster Bio Read more ›
Product Description
Nitric Oxide Synthase 1 (NOS1,neuronal NOS,nNOS1) is a messenger molecule, mediating the effect of endothelium-derived relaxing factor in blood vessels and the cytotoxic actions of macrophages, and playing a part in neuronal communication in the brain. It may be involved in neuronal cell death and damage in neurological illness. nNOS1 localized to the 12q24.2 region of human chromosome 12. It splice variant, expressed in testis, that encodes an NH2-terminal truncated protein of 1098 amino acids. nNOS cDNA clones were shown to contain different 5' terminal exons spliced to a common exon 2. Genomic cloning and sequence analysis demonstrate that the unique exons are positioned within 300 bp of each other but separated from exon 2 by an intron that is at least 20 kb in length. The neuronal isoform of nitric oxide synthase is highly expressed in mammalian skeletal muscle, it suggested a specific role for NOS1 in the local metabolic inhibition of alpha-adrenergic vasoconstriction in active skeletal muscle. The novel gaseous neuromediator nitric oxide is though to play an important role in development and plasticity. Despite this, gene-knockout mice lacking neuronal (Type I) nitric oxide synthase exhibit relatively normal brain development and behavior.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | MA1072 |
| Product category | Primary Antibodies, Mouse Monoclonal Antibodies |
| Reactive species | Goat, Human, Pig, Rat |
| Tested applications | WB |
| Available conjugations | Unconjugated |
| Gene name | NOS1 |
| Protein name | Nitric oxide synthase 1 |
| Specificity | No cross reactivity with other proteins. |
| Immunogen | Recombinant neuronal NOS fragment (amino acids 1-181) from rat brain. |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Purification | Ascites |
| Storage | Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles. |
| UniProt | P29476 |
| NCBI Gene ID | Human(4842), Mouse(18125), Rat(24598) |
Available variants
| Conjugation | Size |
|---|---|
| Unconjugated | 100 μg/vial |
Key Facts
| Target | Nitric oxide synthase 1 |
| Also known as | NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS; Neuronal NOS; Peptidyl-cysteine S-nitrosylase NOS1 |
| Concentration | Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml. |
| Species reactivity | Goat,Human,Pig,Rat |
| Observed band | 200 kDa |
| Purity | No cross reactivity with other proteins. |
| Form / buffer | Lyophilized |
| Shelf life | Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles. |
| Host species | Mouse |
| Clonality | Monoclonal |
| Clone number | Clone: NOS-B1 |
| Isotype | Mouse IgG1 |
| Conjugation | Unconjugated |
| Non-cross-reactive with | No cross-reactivity with other proteins |
| Tested applications |
WB |
| Dilution range | Western blot, 0.5μg/ml, Human, goat, pig, rat |
| Immunogen | Recombinant neuronal NOS fragment (amino acids 1-181) from rat brain. |
| Purification | Ascites |
Reactivity & Application Validation
4 speciess| Species | WB | IHC | IHC-P | IHC-F | IF/ICC | FLOW CYTOMETRY | ELISA | IP |
|---|---|---|---|---|---|---|---|---|
| Goat | ||||||||
| Human | ||||||||
| Pig | ||||||||
| Rat |
Specifications
Storage & Stability
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Compliance & Certifications
Not intended for diagnostic or therapeutic use.