ABM-27078
Anti-NF68 Nefl Antibody (Monoclonal, NR4)
ABMIUM Verified™|Collaborator Tested|Boster Bio
Primary Antibody supplied by Boster Bio Read more ›
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Product Description
Boster Bio Anti-NF68 Nefl Antibody (Monoclonal, NR4) catalog # MA1070. Tested in IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Pig, Rat.
Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 kD (NFL), 125 kD (NFM) and 200 kD (NFH) on SDS-gel electrophoresis. And they have a role in the maturation of regenerating myelinated axons. Neurofilament 68 (NF68), also called Neurofilament Protein, Light Chain (NFL). It is one of the most abundant cytoskeletal components of the neuron. Mutations in this gene were reported as a cause for autosomal dominant Charcot-Marie-Tooth type 2E (CMT2E) linked to chromosome 8p21. NFL was identified repeatedly in both screenings and found to interact with Myotubularin-related 2 gene, MTMR2 in both Schwann cells and neurons.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | MA1070 |
| Product category | Primary Antibodies, Mouse Monoclonal Antibodies |
| Reactive species | Human, Mouse, Pig, Rat |
| Tested applications | IHC, IHC-F, IHC-P, WB |
| Available conjugations | Unconjugated |
| Gene name | NEFL |
| Protein name | Neurofilament light polypeptide |
| Specificity | No cross reactivity with other proteins. |
| Immunogen | Pig spinal cord. |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Purification | Ascites |
| Storage | Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles. |
| UniProt | P19527 |
| NCBI Gene ID | Human(4747), Mouse(18039), Rat(83613) |
Available variants
| Conjugation | Size |
|---|---|
| Unconjugated | 100 μg/vial |
Key Facts
Product type
Primary Antibody›
Target: Neurofilament light polypeptide
| Target | Neurofilament light polypeptide |
| Also known as | NFL; NF-L; NF68; CMT1F; CMT2E; 68 kDa neurofilament protein; Neurofilament triplet L protein |
| Concentration | Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml. |
| Species reactivity | Human,Mouse,Pig,Rat |
| Observed band | 72 kDa |
| Purity | No cross reactivity with other proteins. |
| Form / buffer | Lyophilized |
| Shelf life | Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles. |
| Host species | Mouse |
| Clonality | Monoclonal |
| Clone number | Clone: NR4 |
| Isotype | Mouse IgG1 |
| Conjugation | Unconjugated |
| Non-cross-reactive with | No cross-reactivity with other proteins |
| Tested applications |
IHCIHC-FIHC-PWB |
| Dilution range | Immunohistochemistry (Paraffin-embedded Section), 2-4μg/ml, Human, mouse, pig, rat Immunohistochemistry (Frozen Section), 2-4μg/ml, Human, mouse, pig, rat, - Western blot, 1-2μg/ml, Human, mouse, pig, rat |
| Immunogen | Pig spinal cord. |
| Purification | Ascites |
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Reactivity & Application Validation
4 speciess| Species | WB | IHC | IHC-P | IHC-F | IF/ICC | FLOW CYTOMETRY | ELISA | IP |
|---|---|---|---|---|---|---|---|---|
| Human | ||||||||
| Mouse | ||||||||
| Pig | ||||||||
| Rat |
Specifications
Target
Neurofilament light polypeptide
Also known as
NFL; NF-L; NF68; CMT1F; CMT2E; 68 kDa neurofilament protein; Neurofilament triplet L protein
Concentration
Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
Purity
No cross reactivity with other proteins.
Form / buffer
Lyophilized
Host species
Mouse
Clonality
Monoclonal
Clone number
Clone: NR4
Isotype
Mouse IgG1
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Pig,Rat
Tested applications
IHCIHC-FIHC-PWB
Immunogen
Pig spinal cord.
Observed band
72 kDa
Dilution range
Immunohistochemistry (Paraffin-embedded Section), 2-4μg/ml, Human, mouse, pig, rat
Immunohistochemistry (Frozen Section), 2-4μg/ml, Human, mouse, pig, rat, -
Western blot, 1-2μg/ml, Human, mouse, pig, rat
Immunohistochemistry (Frozen Section), 2-4μg/ml, Human, mouse, pig, rat, -
Western blot, 1-2μg/ml, Human, mouse, pig, rat
Purification
Ascites
Storage & Stability
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Long-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage conditions
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage buffer
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3. *This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Shelf life
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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