Anti-GAPDH Antibody Picoband® (HRP)
Primary Antibody supplied by Boster Bio Read more ›

Product Description
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | A00227-HRP |
| Product category | Primary Antibodies |
| Reactive species | Chicken, Human, Monkey, Mouse, Rat, Zebrafish |
| Tested applications | WB |
| Available conjugations | HRP |
| Gene name | GAPDH |
| Protein name | 40S ribosomal protein S4, X isoform/Y isoform 1/Yisoform 2 |
| Immunogen | HRP-GAPDH antibody was raised against a synthetic peptide containing 16 amino acids near the carboxy terminus of GAPDH. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification | HRP-GAPDH antibody is affinity chromatography purified via peptide column. |
| Storage | HRP-GAPDH antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| UniProt | P04406 |
| NCBI Gene ID | 2597 |
Available variants
| Conjugation | Size |
|---|---|
| HRP | 100 μg/vial |
Key Facts
| Target | 40S ribosomal protein S4, X isoform/Y isoform 1/Yisoform 2 |
| Also known as | G3PD; GAPD; GAPDH; OK/SW cl.12 |
| Concentration | 0.5 mg/mL |
| Species reactivity | Chicken,Human,Monkey,Mouse,Rat,Zebrafish |
| Observed band | 36 kDa |
| Form / buffer | Liquid |
| Shelf life | HRP-GAPDH antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | HRP |
| Non-cross-reactive with | HRP-GAPDH antibody is human, mouse, rat, rabbit, and chicken reactive. |
| Tested applications |
WB |
| Dilution range | Western blot, 0.02-0.1 μg/ml |
| Immunogen | HRP-GAPDH antibody was raised against a synthetic peptide containing 16 amino acids near the carboxy terminus of GAPDH. |
| Purification | HRP-GAPDH antibody is affinity chromatography purified via peptide column. |
Reactivity & Application Validation
6 speciess| Species | WB | IHC | IHC-P | IHC-F | IF/ICC | FLOW CYTOMETRY | ELISA | IP |
|---|---|---|---|---|---|---|---|---|
| Chicken | ||||||||
| Human | ||||||||
| Monkey | ||||||||
| Mouse | ||||||||
| Rat | ||||||||
| Zebrafish |
Specifications
Storage & Stability
Compliance & Certifications
Not intended for diagnostic or therapeutic use.