Anti-Carbonic Anhydrase 9/CA9 Antibody Picoband®
Primary Antibody supplied by Boster Bio Read more ›

Product Description
Carbonic anhydrase IX (CA9/CA IX) is an enzyme that in humans is encoded by the CA9 gene. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | A01083-3 |
| Product category | Primary Antibodies |
| Reactive species | Human, Mouse |
| Tested applications | ELISA, Flow Cytometry, IHC, IHC-P, WB |
| Available conjugations | Unconjugated, Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, Fluoro647 |
| Gene name | CA9 |
| Protein name | Carbonic anhydrase 9 |
| Immunogen | E.coli-derived human Carbonic Anhydrase 9/CA9 recombinant protein (Position: D146-D414). |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Purification | Immunogen affinity purified. |
| Storage | At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing. |
| UniProt | Q16790 |
| NCBI Gene ID | 768 |
Available variants
| Conjugation | Size |
|---|---|
| Unconjugated | 100 μg/vial |
| Biotin | 100 μg/vial |
| Cy3 | 100 μg/vial |
| Fluoro488 | 100 μg/vial |
| Fluoro550 | 100 μg/vial |
| Fluoro594 | 100 μg/vial |
| FITC | 100 μg/vial |
| HRP | 100 μg/vial |
| APC | 100 μg/vial |
| PE | 100 μg/vial |
| Fluoro647 | 100 μg/vial |
Key Facts
| Target | Carbonic anhydrase 9 |
| Also known as | CA IX; CA9; CAIX; Carbonate dehydratase IX; Carbonic anhydrase 9; carbonic anhydrase IX; G250; Membrane antigen MN; MN; P54/58N; pMW1; RCC associated antigen G250 |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Species reactivity | Human,Mouse |
| Observed band | 54 kDa |
| Form / buffer | Lyophilized |
| Shelf life | At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing. |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647 |
| Non-cross-reactive with | No cross-reactivity with other proteins. |
| Tested applications |
ELISAFlow CytometryIHCIHC-PWB |
| Dilution range | Western blot, 0.25-0.5 μg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human, Mouse ELISA, 0.1-0.5 μg/ml, - |
| Immunogen | E.coli-derived human Carbonic Anhydrase 9/CA9 recombinant protein (Position: D146-D414). |
| Purification | Immunogen affinity purified. |
Reactivity & Application Validation
2 speciess| Species | WB | IHC | IHC-P | IHC-F | IF/ICC | FLOW CYTOMETRY | ELISA | IP |
|---|---|---|---|---|---|---|---|---|
| Human | ||||||||
| Mouse |
Specifications
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human, Mouse
ELISA, 0.1-0.5 μg/ml, -
Storage & Stability
Compliance & Certifications
Not intended for diagnostic or therapeutic use.