Anti-Ataxin 3/ATXN3 Antibody Picoband®

Boster Bio Anti-Ataxin 3/ATXN3 Antibody Picoband® catalog # PB9423. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

ATXN3 (Ataxin 3), also known as AT3, MJD GENE, MJD1, SCA3 GENE, ATX3, JOS, Spinocerebellar ataxia-3, Machado-Joseph disease protein 1, is a protein that in humans is encoded by the ATXN3 gene. ATXN3 ranges in size from 360 to 374 amino acids. Using Northern blot analysis showed that ATXN3 mRNA was ubiquitously expressed in human tissues. They detected at least 4 ATXN3 transcripts of 1.4, 1.8, 4.5, and 7.5 kb and suggested that the different mRNA species probably result from differential splicing and polyadenylation. Machado-Joseph disease, also known as spinocerebellar ataxia-3, is an autosomal dominant neurologic disorder. The protein encoded by the ATXN3 gene contains (CAG)n repeats in the coding region, and the expansion of these repeats from the normal 13-36 to 68-79 is the cause of Machado-Joseph disease. There is an inverse correlation between the age of onset and CAG repeat numbers. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. Ataxin-3 interacted with 2 human homologs of the yeast DNA repair protein RAD23, HHR23A (RAD23A) and HHR23B (RAD23B). Both normal and mutant ataxin-3 proteins interacted with the ubiquitin-like domain at the N terminus of the HHR23 proteins, which is a motif important for nucleotide excision repair. However, in HEK 293 cells, HHR23A was recruited to intranuclear inclusions formed by the mutant ataxin-3 through its interaction with ataxin-3.

Product information

Supplier	Boster Bio
ABMIUM evidence status	ABMIUM Verified™ collaborator-tested data
Catalogue reference	PB9423
Product category	Primary Antibodies
Reactive species	Human, Rat
Tested applications	ELISA, Flow Cytometry, IF/ICC, IHC, IHC-P, WB
Available conjugations	Unconjugated, Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, Fluoro647
Gene name	ATXN3
Protein name	Ataxin-3
Specificity	No cross reactivity with other proteins.
Immunogen	A synthetic peptide corresponding to a sequence at the C-terminus of human Ataxin 3, different from the related mouse and rat sequences by two amino acids.
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Purification	Immunogen affinity purified.
Storage	Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
UniProt	P54252
NCBI Gene ID	4287

Available variants

Conjugation	Size
Unconjugated	100 μg/vial
Biotin	100 μg/vial
Cy3	100 μg/vial
Fluoro488	100 μg/vial
Fluoro550	100 μg/vial
Fluoro594	100 μg/vial
FITC	100 μg/vial
HRP	100 μg/vial
APC	100 μg/vial
PE	100 μg/vial
Fluoro647	100 μg/vial