Primary Antibody ABMIUM Verified™Collaborator Tested
ABM-29297

Anti-AKT1 Monoclonal Antibody

ABMIUM Verified™|Collaborator Tested|Boster Bio

Primary Antibody supplied by Boster Bio Read more ›

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Anti-AKT1 Monoclonal Antibody Boster Bio
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Anti-AKT1 Monoclonal Antibody Boster Bio

Product Description

Boster Bio Anti-AKT1 Monoclonal Antibody catalog # M00024-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product information

Supplier Boster Bio
ABMIUM evidence status ABMIUM Verified™ collaborator-tested data
Catalogue reference M00024-1
Product category Primary Antibodies, Rabbit Monoclonal Antibodies
Reactive species Human, Mouse, Rat
Tested applications Flow Cytometry, IF/ICC, IHC, IP, WB
Available conjugations Unconjugated
Gene name AKT1
Protein name RAC-alpha serine/threonine-protein kinase
Immunogen A synthesized peptide derived from human AKT1 Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.
Host Rabbit
Clonality Monoclonal
Isotype Rabbit IgG
Purification Affinity-chromatography
Storage Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
UniProt P31749
NCBI Gene ID 207

Available variants

Conjugation Size
Unconjugated 100 μl/vial

Key Facts

Product type Primary Antibody Target: RAC-alpha serine/threonine-protein kinase
Target RAC-alpha serine/threonine-protein kinase
Also known as AKT; AKT1; PKB; PKB ALPHA; PRKBA; Protein kinase B; Proto oncogene c Akt; RAC; RAC ALPHA; RAC PK alpha
Concentration 0.5mg/ml
Species reactivity Human,Mouse,Rat
Observed band 56 kDa
Form / buffer Liquid
Shelf life Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Host species Rabbit
Clonality Monoclonal
Clone number Clone: EBG-1
Isotype Rabbit IgG
Conjugation Unconjugated
Tested applications
Flow CytometryIF/ICCIHCIPWB
Dilution range WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
FC 1:50
Immunogen A synthesized peptide derived from human AKT1 Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.
Purification Affinity-chromatography
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Reactivity & Application Validation

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFLOW CYTOMETRYELISAIP
Human
Mouse
Rat

Specifications

Target
RAC-alpha serine/threonine-protein kinase
Also known as
AKT; AKT1; PKB; PKB ALPHA; PRKBA; Protein kinase B; Proto oncogene c Akt; RAC; RAC ALPHA; RAC PK alpha
Concentration
0.5mg/ml
Form / buffer
Liquid
Host species
Rabbit
Clonality
Monoclonal
Clone number
Clone: EBG-1
Isotype
Rabbit IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
Flow CytometryIF/ICCIHCIPWB
Immunogen
A synthesized peptide derived from human AKT1 Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.
Observed band
56 kDa
Dilution range
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
FC 1:50
Purification
Affinity-chromatography

Storage & Stability

Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Long-term storage
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Storage conditions
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Storage buffer
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Shelf life
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

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