Recombinant MERS-CoV Spike Protein (S1+S2 ECD, aa 1-1297, His Tag)
Recombinant MERS-CoV Spike Protein (S1+S2 ECD, aa 1-1297, His Tag) supplied by Elabscience with collaborator-provided technical specifications. Read more ›
["Measured by its binding ability in a functional ELISA. 1. Recombinant MERS-CoV Spike Protein (S1+S2 ECD, aa 1-1297, His Tag)(Cat:PKSV030236) at 10 μg/mL (100 μl/well) can bind biotinylated DPP4 (Cat:PKSH033811), The EC50 of can biotinylated DPP4 (Cat:PKSH033811) is 0.02-0.04 ug/mL. 2. Recombinant MERS-CoV Spike Protein (S1+S2 ECD, aa 1-1297, His Tag)(Cat:PKSV030236) at 10 μg/mL (100 μl/well) can bind biotinylated Fc-DPP4 (Cat:PKSH030456), The EC50 of can biotinylated Fc-DPP4 (Cat:PKSH030456) is 0.01-0.02 ug/mL. ,"]Product Description
MERS-CoV Spike is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.
Protein profile: reactive species/source: MERS-CoV; expression system: Baculovirus-Insect Cells; physical form: Lyophilized from sterile 20mM Tris, 500mM Nacl, pH 7.4, 10% glycerol.<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.; tag: MERS-CoV ,MERS-CoV Spike ,Recombinant Proteins; predicted molecular weight: 142.5 kDa.
Quality and formulation: purity: > 85 % as determined by reducing SDS-PAGE.; endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method..
Functional evidence: activity: Measured by its binding ability in a functional ELISA. 1. Recombinant MERS-CoV Spike Protein (S1+S2 ECD, aa 1-1297, His Tag)(Cat:PKSV030236) at 10 μg/mL (100 μl/well) can bind biotinylated DPP4 (Cat:PKSH033811), The EC<sub>50</sub> of can biotinylated DPP4 (Cat:PKSH033811) is 0.02-0.04 ug/mL. 2. Recombinant MERS-CoV Spike Protein (S1+S2 ECD, aa 1-1297, His Tag)(Cat:PKSV030236) at 10 μg/mL (100 μl/well) can bind biotinylated Fc-DPP4 (Cat:PKSH030456), The EC<sub>50</sub> of can biotinylated Fc-DPP4 (Cat:PKSH030456) is 0.01-0.02 ug/mL.. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.
Sequence coverage: 142.5 kDa.
Database references: UniProt: AFS88936.1 | Gene symbol: MERS-CoV Spike.
Research Use Only.
Key Facts
| Target | MERS-CoV Spike |
| Also known as | MERS-CoV, RBD Protein, S Pr, coronavirus s1 Protein, coronavirus s2 Protein, coronavirus spike Protein, cov spike Protein, ncov RBD Protein, ncov s1 Protein, ncov s2 Protein, ncov spike Protein |
| Species reactivity | MERS-CoV |
| Purity | > 85 % as determined by reducing SDS-PAGE. |
| Form / buffer | Lyophilized from sterile 20mM Tris, 500mM Nacl, pH 7.4, 10% glycerol. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Shelf life | 12 months |
| BIOACTIVITY | BINDING | CELL-BASED | IN VIVO | ELISA CAPTURE | |
|---|---|---|---|---|---|
| MERS-CoV |
Specifications
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Manufactured under ISO 14001:2015 quality management standards.
Manufactured under ISO 45001:2018 quality management standards.
Not intended for diagnostic or therapeutic use.
Substance does not appear on the SVHC candidate list.