Recombinant Proteins ISO 9001:2015ISO 14001:2015ISO 45001:2018ABMIUM Verified™Collaborator Tested
ABM-20365

Recombinant MERS-CoV Spike Protein (S1+S2 ECD, aa 1-1297, His Tag)

Measured by its binding ability in a functional ELISA. 1. Recombinant MERS-CoV Spike Protein (S1+S2 ECDaa 1-1297His Tag)(Cat:PKSV030236) at 10 μg/mL (100 μl/well) can bind biotinylated DPP4 (Cat:PKSH033811)The EC50 of can biotinylated DPP4 (Cat:PKSH033811) is 0.02-0.04 ug/mL. 2. Recombinant MERS-CoV Spike Protein (S1+S2 ECDaa 1-1297His Tag)(Cat:PKSV030236) at 10 μg/mL (100 μl/well) can bind biotinylated Fc-DPP4 (Cat:PKSH030456)The EC50 of can biotinylated Fc-DPP4 (Cat:PKSH030456) is 0.01-0.02 ug/mL.Baculovirus-Insect Cells< 1.0 EU per μg of the protein as determined by the LAL method.

Recombinant MERS-CoV Spike Protein (S1+S2 ECD, aa 1-1297, His Tag) supplied by Elabscience with collaborator-provided technical specifications. Read more ›

Related formats
Measured by its binding ability in a functional ELISA.1. Immobilized Sh(2C-EMC)(1-1297)(Cat: PKSV030236) at 10 μg/mL (100 μl/well) can bind biotinylated DPP4 (Cat: PKSH033811), The EC<sub>50</sub> of can biotinylated DPP4 (Cat: PKSH033811) is 0.02-0.04 μg/mL.2. Immobilized Sh(2C-EMC)(1-1297)(Cat: PKSV030236) at 10 μg/mL (100 μl/well) can bind biotinylated Fc-DPP4 (Cat: PKSH030456), The EC<sub>50</sub> of can biotinylated Fc-DPP4 (Cat: PKSH030456) is 0.01-0.02 μg/mL.
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Measured by its binding ability in a functional ELISA.1. Immobilized Sh(2C-EMC)(1-1297)(Cat: PKSV030236) at 10 μg/mL (100 μl/well) can bind biotinylated DPP4 (Cat: PKSH033811), The EC<sub>50</sub> of can biotinylated DPP4 (Cat: PKSH033811) is 0.02-0.04 μg/mL.2. Immobilized Sh(2C-EMC)(1-1297)(Cat: PKSV030236) at 10 μg/mL (100 μl/well) can bind biotinylated Fc-DPP4 (Cat: PKSH030456), The EC<sub>50</sub> of can biotinylated Fc-DPP4 (Cat: PKSH030456) is 0.01-0.02 μg/mL.["Measured by its binding ability in a functional ELISA. 1. Recombinant MERS-CoV Spike Protein (S1+S2 ECD, aa 1-1297, His Tag)(Cat:PKSV030236) at 10 μg/mL (100 μl/well) can bind biotinylated DPP4 (Cat:PKSH033811), The EC50 of can biotinylated DPP4 (Cat:PKSH033811) is 0.02-0.04 ug/mL. 2. Recombinant MERS-CoV Spike Protein (S1+S2 ECD, aa 1-1297, His Tag)(Cat:PKSV030236) at 10 μg/mL (100 μl/well) can bind biotinylated Fc-DPP4 (Cat:PKSH030456), The EC50 of can biotinylated Fc-DPP4 (Cat:PKSH030456) is 0.01-0.02 ug/mL. ,"]

Product Description

MERS-CoV Spike is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.

Protein profile: reactive species/source: MERS-CoV; expression system: Baculovirus-Insect Cells; physical form: Lyophilized from sterile 20mM Tris, 500mM Nacl, pH 7.4, 10% glycerol.<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.; tag: MERS-CoV ,MERS-CoV Spike ,Recombinant Proteins; predicted molecular weight: 142.5 kDa.

Quality and formulation: purity: > 85 % as determined by reducing SDS-PAGE.; endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method..

Functional evidence: activity: Measured by its binding ability in a functional ELISA. 1. Recombinant MERS-CoV Spike Protein (S1+S2 ECD, aa 1-1297, His Tag)(Cat:PKSV030236) at 10 μg/mL (100 μl/well) can bind biotinylated DPP4 (Cat:PKSH033811), The EC<sub>50</sub> of can biotinylated DPP4 (Cat:PKSH033811) is 0.02-0.04 ug/mL. 2. Recombinant MERS-CoV Spike Protein (S1+S2 ECD, aa 1-1297, His Tag)(Cat:PKSV030236) at 10 μg/mL (100 μl/well) can bind biotinylated Fc-DPP4 (Cat:PKSH030456), The EC<sub>50</sub> of can biotinylated Fc-DPP4 (Cat:PKSH030456) is 0.01-0.02 ug/mL.. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.

Sequence coverage: 142.5 kDa.

Database references: UniProt: AFS88936.1 | Gene symbol: MERS-CoV Spike.

Research Use Only.

Key Facts

Product type Recombinant Proteins Target: MERS-CoV Spike
Target MERS-CoV Spike
Also known as MERS-CoV, RBD Protein, S Pr, coronavirus s1 Protein, coronavirus s2 Protein, coronavirus spike Protein, cov spike Protein, ncov RBD Protein, ncov s1 Protein, ncov s2 Protein, ncov spike Protein
Species reactivity MERS-CoV
Purity > 85 % as determined by reducing SDS-PAGE.
Form / buffer Lyophilized from sterile 20mM Tris, 500mM Nacl, pH 7.4, 10% glycerol.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Shelf life 12 months
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1
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
BIOACTIVITYBINDINGCELL-BASEDIN VIVOELISA CAPTURE
MERS-CoV

Specifications

Target
MERS-CoV Spike
Also known as
MERS-CoV, RBD Protein, S Pr, coronavirus s1 Protein, coronavirus s2 Protein, coronavirus spike Protein, cov spike Protein, ncov RBD Protein, ncov s1 Protein, ncov s2 Protein, ncov spike Protein
Purity
> 85 % as determined by reducing SDS-PAGE.
Form / buffer
Lyophilized from sterile 20mM Tris, 500mM Nacl, pH 7.4, 10% glycerol.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Storage & Stability

Shipped at
This product is provided as lyophilized powder which is shipped with ice packs.
Short-term storage
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Long-term storage
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Aliquoting advice
Reconstitute entire vial immediately. Aliquot working stock; avoid repeated freeze-thaw.
Shelf life
12 months

Compliance & Certifications

ISO 9001:2015
ISO 9001:2015 Certified

Manufactured under ISO 9001:2015 quality management standards.

ISO 14001:2015
ISO 14001:2015 Certified

Manufactured under ISO 14001:2015 quality management standards.

ISO 45001:2018
ISO 45001:2018 Certified

Manufactured under ISO 45001:2018 quality management standards.

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

REACH
REACH Compliant

Substance does not appear on the SVHC candidate list.

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