Recombinant Proteins ISO 9001:2015ISO 14001:2015ISO 45001:2018ABMIUM Verified™Collaborator Tested
ABM-20215

Recombinant SARS-CoV-2 Spike Protein (RBD, His Tag)(G476S)

Recombinant Human ACE2 Protein (mFc Tag)(Active)(Cat#PKSR030509) at 2 μg/mL (100 μL/well) can bind Recombinant 2019-nCoV Spike Protein (RBDHis Tag)(G476S)(Active)(Cat#PKSR030533)the EC50 of PKSR030533 is 25-60 ng/mL.HEK293 Cells< 1.0 EU per μg of the protein as determined by the LAL method.

Recombinant SARS-CoV-2 Spike Protein (RBD, His Tag)(G476S) supplied by Elabscience with collaborator-provided technical specifications. Read more ›

Related formats
Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag)(Cat: PKSH031870) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD(G476S)-His Recombinant Protein (PKSR030533), the EC<sub>50</sub> of SARS-CoV-2 (2019-nCoV) Spike RBD(G476S)-His Recombinant Protein (Cat: PKSR0305198) is 25-60 ng/mL.
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Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag)(Cat: PKSH031870) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD(G476S)-His Recombinant Protein (PKSR030533), the EC<sub>50</sub> of SARS-CoV-2 (2019-nCoV) Spike RBD(G476S)-His Recombinant Protein (Cat: PKSR0305198) is 25-60 ng/mL.["Recombinant Human ACE2 Protein (mFc Tag)(Active)(Cat#PKSR030509) at 2 μg/mL (100 μL/well) can bind Recombinant 2019-nCoV Spike Protein (RBD, His Tag)(G476S)(Active)(Cat#PKSR030533), the EC50 of PKSR030533 is 25-60 ng/mL. ,"]

Product Description

SARS-CoV-2 Spike(RBD)(G476S) is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.

Protein profile: reactive species/source: SARS-CoV-2; expression system: HEK293 Cells; physical form: Lyophilized from sterile PBS,pH 7.4<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.; tag: SARS-CoV-2 ,SARS-CoV-2 Spike(RBD)(G476S) ,Recombinant Proteins; predicted molecular weight: 26.6 kDa.

Quality and formulation: purity: > 95 % as determined by reducing SDS-PAGE.; endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method..

Functional evidence: activity: Recombinant Human ACE2 Protein (mFc Tag)(Active)(Cat#PKSR030509) at 2 μg/mL (100 μL/well) can bind Recombinant 2019-nCoV Spike Protein (RBD, His Tag)(G476S)(Active)(Cat#PKSR030533), the EC<sub>50</sub> of PKSR030533 is 25-60 ng/mL.. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.

Sequence coverage: 26.6 kDa.

Database references: UniProt: YP_009724390.1 | Gene symbol: SARS-CoV-2 Spike(RBD)(G476S).

Research Use Only.

Key Facts

Product type Recombinant Proteins Target: SARS-CoV-2 Spike(RBD)(G476S)
Target SARS-CoV-2 Spike(RBD)(G476S)
Also known as NCP-CoV RBD, NCP-CoV Spike, NCP-CoV s1, NCP-CoV s2, RBD, S1, S2, Spike RBD, coronavirus spike, cov spike, ncov RBD, ncov s1, ncov s2, ncov spike, novel coronavirus RBD, novel coronavirus s1, novel coronavirus s2, novel coronavirus spike
Species reactivity SARS-CoV-2
Purity > 95 % as determined by reducing SDS-PAGE.
Form / buffer Lyophilized from sterile PBS,pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Shelf life 12 months
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ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
BIOACTIVITYBINDINGCELL-BASEDIN VIVOELISA CAPTURE
SARS-CoV-2

Specifications

Target
SARS-CoV-2 Spike(RBD)(G476S)
Also known as
NCP-CoV RBD, NCP-CoV Spike, NCP-CoV s1, NCP-CoV s2, RBD, S1, S2, Spike RBD, coronavirus spike, cov spike, ncov RBD, ncov s1, ncov s2, ncov spike, novel coronavirus RBD, novel coronavirus s1, novel coronavirus s2, novel coronavirus spike
Purity
> 95 % as determined by reducing SDS-PAGE.
Form / buffer
Lyophilized from sterile PBS,pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Storage & Stability

Shipped at
This product is provided as lyophilized powder which is shipped with ice packs.
Short-term storage
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Long-term storage
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Aliquoting advice
Reconstitute entire vial immediately. Aliquot working stock; avoid repeated freeze-thaw.
Shelf life
12 months

Compliance & Certifications

ISO 9001:2015
ISO 9001:2015 Certified

Manufactured under ISO 9001:2015 quality management standards.

ISO 14001:2015
ISO 14001:2015 Certified

Manufactured under ISO 14001:2015 quality management standards.

ISO 45001:2018
ISO 45001:2018 Certified

Manufactured under ISO 45001:2018 quality management standards.

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

REACH
REACH Compliant

Substance does not appear on the SVHC candidate list.

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