Recombinant Proteins ISO 9001:2015ISO 14001:2015ISO 45001:2018ABMIUM Verified™Collaborator Tested
ABM-16629

Recombinant Human CD32b/FCGR2B Protein (His &AVI Tag), Biotinylated

Measured by its binding ability in a functional ELISA. Immobilized Human IgG1 at 10 μg/ml (100 μl/well) can bind Human CD32b. The EC50 of Human CD32b is 1. 6 - 3. 7 μg/ml. 2. Labeling ratio of biotin to protein: 0.5HEK293 Cells< 1.0 EU per μg of the protein as determined by the LAL method.

Recombinant Human CD32b/FCGR2B Protein (His &AVI Tag), Biotinylated supplied by Elabscience with collaborator-provided technical specifications. Read more ›

Related formats
Loaded Biotinylated Human FcγRIIB/C / CD32b/c protein (His & Avi Tag) (Cat. PKSH031724) on SA Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 1.2 μM as determined in a BLI assay (Sartorius Octet Red384).
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Loaded Biotinylated Human FcγRIIB/C / CD32b/c protein (His & Avi Tag) (Cat. PKSH031724) on SA Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 1.2 μM as determined in a BLI assay (Sartorius Octet Red384).["Measured by its binding ability in a functional ELISA. Immobilized Human IgG1 at 10 μg/ml (100 μl/well) can bind Human CD32b. The EC50 of Human CD32b is 1. 6 - 3. 7 μg/ml. 2. Labeling ratio of biotin to protein: 0.5 ,"]

Product Description

CD32b/FCGR2B is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.

Protein profile: reactive species/source: Human; expression system: HEK293 Cells; physical form: Lyophilized from sterile PBS, pH 7.4<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.; tag: Human ,CD32b/FCGR2B ,Recombinant Proteins; predicted molecular weight: 24 kDa.

Quality and formulation: purity: > 95 % as determined by reducing SDS-PAGE.; endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method..

Functional evidence: activity: Measured by its binding ability in a functional ELISA. Immobilized Human IgG1 at 10 μg/ml (100 μl/well) can bind Human CD32b. The EC<sub>50</sub> of Human CD32b is 1. 6 - 3. 7 μg/ml. 2. Labeling ratio of biotin to protein: 0.5. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.

Sequence coverage: 24 kDa.

Database references: UniProt: NP_001002274.1 | NCBI Gene: 2213 | Gene symbol: CD32b/FCGR2B.

Research Use Only.

Key Facts

Product type Recombinant Proteins Target: CD32b/FCGR2B
Target CD32b/FCGR2B
Also known as CD32, CDw32, FCG2, FCGR2B, Fc-Gamma RII-b, Fc-Gamma-RIIb, FcRII-b, IGFR2, IgG Fc Receptor II-b, Low Affinity Immunoglobulin Gamma Fc Region Receptor II-b
Species reactivity Human
Purity > 95 % as determined by reducing SDS-PAGE.
Form / buffer Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Shelf life 12 months
Research area immunology, Stem cells
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ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
BIOACTIVITYBINDINGCELL-BASEDIN VIVOELISA CAPTURE
Human

Specifications

Target
CD32b/FCGR2B
Also known as
CD32, CDw32, FCG2, FCGR2B, Fc-Gamma RII-b, Fc-Gamma-RIIb, FcRII-b, IGFR2, IgG Fc Receptor II-b, Low Affinity Immunoglobulin Gamma Fc Region Receptor II-b
Purity
> 95 % as determined by reducing SDS-PAGE.
Form / buffer
Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Storage & Stability

Shipped at
This product is provided as lyophilized powder which is shipped with ice packs.
Short-term storage
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Long-term storage
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Aliquoting advice
Reconstitute entire vial immediately. Aliquot working stock; avoid repeated freeze-thaw.
Shelf life
12 months

Compliance & Certifications

ISO 9001:2015
ISO 9001:2015 Certified

Manufactured under ISO 9001:2015 quality management standards.

ISO 14001:2015
ISO 14001:2015 Certified

Manufactured under ISO 14001:2015 quality management standards.

ISO 45001:2018
ISO 45001:2018 Certified

Manufactured under ISO 45001:2018 quality management standards.

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

REACH
REACH Compliant

Substance does not appear on the SVHC candidate list.

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