Recombinant Human R-Spondin 1/RSPO1 Protein (His Tag)
Recombinant Human R-Spondin 1/RSPO1 Protein (His Tag) supplied by Elabscience with collaborator-provided technical specifications. Read more ›
["1. Immobilized human RSPO1 at 20 μg/ml (100 μl/well) can bind human LIMPII with a linear ranger of 32-800 ng/ml. 2. Immobilized human RSPO1 at 20 μg/ml (100 μl/well) can bind mouse CD36 with a linear ranger of 6. 4-800 ng/ml. 3. Measured by its ability to induce activation of ßcatenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.1-0.9 μg/mL in the presence of 5 ng/mL recombinant mouse Wnt3a. ,42 kDa"]Product Description
R-Spondin 1/RSPO1 is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.
Protein profile: reactive species/source: Human; expression system: HEK293 Cells; physical form: Lyophilized from sterile PBS, pH 7.4<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.; tag: Human ,R-Spondin 1/RSPO1 ,Recombinant Proteins; predicted molecular weight: 28.2 kDa.
Quality and formulation: purity: > 95 % as determined by reducing SDS-PAGE.; endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method..
Functional evidence: activity: 1. Immobilized human RSPO1 at 20 μg/ml (100 μl/well) can bind human LIMPII with a linear ranger of 32-800 ng/ml. 2. Immobilized human RSPO1 at 20 μg/ml (100 μl/well) can bind mouse CD36 with a linear ranger of 6. 4-800 ng/ml. 3. Measured by its ability to induce activation of ßcatenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED<sub>50</sub> for this effect is typically 0.1-0.9 μg/mL in the presence of 5 ng/mL recombinant mouse Wnt3a.. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.
Sequence coverage: 28.2 kDa.
Database references: UniProt: NP_001033722.1 | NCBI Gene: 284654 | Gene symbol: R-Spondin 1/RSPO1.
Research Use Only.
Key Facts
| Target | R-Spondin 1/RSPO1 |
| Also known as | CRISTIN3, FLJ40906, R-spondin, R-spondin1, RP11-566C13.1, RP23-325M14.2, RSPO Rspo1, RSPO1, Roof plate-specific spondin-1, Rspondin |
| Species reactivity | Human |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Form / buffer | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Shelf life | 12 months |
| Research area | Developmental Biology, Stem cells |
| BIOACTIVITY | BINDING | CELL-BASED | IN VIVO | ELISA CAPTURE | |
|---|---|---|---|---|---|
| Human |
Specifications
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Manufactured under ISO 14001:2015 quality management standards.
Manufactured under ISO 45001:2018 quality management standards.
Not intended for diagnostic or therapeutic use.
Substance does not appear on the SVHC candidate list.