Recombinant Human CD31/PECAM1 Protein (Fc Tag)
Recombinant Human CD31/PECAM1 Protein (Fc Tag) supplied by Elabscience with collaborator-provided technical specifications. Read more ›
["Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8 × 104 cells/well are added to sCD31-Fc coated plates (1. 25μg/mL, 100μL/well) in the presence of 20ng/mL PMA, approximately 20-45% will adhere after 30 minutes at 37℃. ,130-140 kDa"]Product Description
CD31/PECAM1 is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.
Protein profile: reactive species/source: Human; expression system: HEK293 Cells; physical form: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.; tag: Human ,CD31/PECAM1 ,Recombinant Proteins; predicted molecular weight: 91.2 kDa.
Quality and formulation: purity: > 95 % as determined by reducing SDS-PAGE.; endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method..
Functional evidence: activity: Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8 × 10<sup>4</sup> cells/well are added to sCD31-Fc coated plates (1. 25μg/mL, 100μL/well) in the presence of 20ng/mL PMA, approximately 20-45% will adhere after 30 minutes at 37℃.. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.
Sequence coverage: 91.2 kDa.
Database references: UniProt: EAW94208.1 | NCBI Gene: 5175 | Gene symbol: CD31/PECAM1.
Research Use Only.
Key Facts
| Target | CD31/PECAM1 |
| Also known as | CD31, EndoCAM, GPIIA, PECA1, PECAM-1, PECAM1 , Platelet endothelial cell adhesion molecule |
| Species reactivity | Human |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Form / buffer | Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Shelf life | 12 months |
| Research area | Cardiovascular, Signal Transduction, Stem Cells, Cancer |
| BIOACTIVITY | BINDING | CELL-BASED | IN VIVO | ELISA CAPTURE | |
|---|---|---|---|---|---|
| Human |
Specifications
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Manufactured under ISO 14001:2015 quality management standards.
Manufactured under ISO 45001:2018 quality management standards.
Not intended for diagnostic or therapeutic use.
Substance does not appear on the SVHC candidate list.