Recombinant Human IL-23(IL23A&IL12B Heterodimer) Protein (His Tag)
Recombinant Human IL-23(IL23A&IL12B Heterodimer) Protein (His Tag) supplied by Elabscience with collaborator-provided technical specifications. Read more ›
["1. Measured by its ability to bind biotinylated recombinant human IL12RB1 in a functional ELISA. 2. Immobilized human IL23A-His+IL12B-His at 10 μg/ml (100 μl/well) can bind human IL23R-Fc. The EC50 of human IL23R-Fc is 0.28-0.66 μg/ml. 3. Immobilized human IL23A-His+IL12B-His at 10 μg/ml (100 μl/well) can bind Cynomolgus IL23R-Fc. The EC50 of Cynomolgus IL23R-Fc is 0.14-0.35 μg/ml. 4. Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 4-20 ng/mL. ,22&45 kDa"]Product Description
IL-23(IL23A&IL12B is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.
Protein profile: reactive species/source: Human; expression system: HEK293 Cells; physical form: Lyophilized from sterile PBS, pH 7.4<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.; tag: Human ,IL-23(IL23A&IL12B ,Cytokines; predicted molecular weight: 20.1&36.2 kDa.
Quality and formulation: purity: > 90 % as determined by reducing SDS-PAGE.; endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method..
Functional evidence: activity: 1. Measured by its ability to bind biotinylated recombinant human IL12RB1 in a functional ELISA. 2. Immobilized human IL23A-His+IL12B-His at 10 μg/ml (100 μl/well) can bind human IL23R-Fc. The EC<sub>50</sub> of human IL23R-Fc is 0.28-0.66 μg/ml. 3. Immobilized human IL23A-His+IL12B-His at 10 μg/ml (100 μl/well) can bind Cynomolgus IL23R-Fc. The EC<sub>50</sub> of Cynomolgus IL23R-Fc is 0.14-0.35 μg/ml. 4. Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED<sub>50</sub> for this effect is 4-20 ng/mL.. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.
Sequence coverage: 20.1&36.2 kDa.
Database references: UniProt: Q9NPF7&NP_002178.2 | Gene symbol: IL-23(IL23A&IL12B.
Research Use Only.
Key Facts
| Target | IL-23(IL23A&IL12B |
| Also known as | CLMF p40, IL-12 subunit p40, IL-23, IL-23A, IL-23p19, IL23P19, NKSF2, P19, SGRF |
| Species reactivity | Human |
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Form / buffer | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Shelf life | 12 months |
| Research area | Microbiology, Cancer, immunology |
| BIOACTIVITY | BINDING | CELL-BASED | IN VIVO | ELISA CAPTURE | |
|---|---|---|---|---|---|
| Human |
Specifications
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Manufactured under ISO 14001:2015 quality management standards.
Manufactured under ISO 45001:2018 quality management standards.
Not intended for diagnostic or therapeutic use.
Substance does not appear on the SVHC candidate list.