Manufactured by:ELK Biotechnology
RUO

VCP (phospho Ser352) rabbit pAb

VCP (phospho Ser352) rabbit pAb from ELK Biotechnology

SKU:
ABM109351
Product type
Primary Antibody
TargetVCP
ApplicationsWB, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemBrain,Epithelium,Fetal brain cortex,Kidney,Lymph,PCR rescued clones,Pituitary,Plate
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

VCP (phospho Ser352) rabbit pAb is a research-use antibody from ELK Biotechnology for studying VCP in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

valosin containing protein(VCP) Homo sapiens The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity Phospho-VCP (S352) Polyclonal Antibody detects endogenous levels of VCP protein only when phosphorylated at S352.
Validation evidence captions Western blot analysis of 22RV1 using p-VCP (S352) antibody. Antibody was diluted at 1:1000 | Western blot analysis of lysates from NIH/3T3 cells treated with starved 24h, using VCP (Phospho-Ser352) Antibody. The lane on the right is blocked with the phospho peptide.
Key facts & specifications
Research areas
>>Protein processing in endoplasmic reticulum, >>Amyotrophic lateral sclerosis, >>Pathways of neurodegeneration - multiple diseases, >>Legionellosis
Also known as
VCP; Transitional endoplasmic reticulum ATPase; TER ATPase; 15S Mg(2+)-ATPase p97 subunit; Valosin-containing protein; VCP
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human VCP around the phosphorylation site of Ser352. AA range:318-367
Observed band
85kD
Cellular localization
Cytoplasm, cytosol . Endoplasmic reticulum . Nucleus . Cytoplasm, Stress granule . Present in the neuronal hyaline inclusion bodies specifically found in motor neurons from amyotrophic lateral sclerosis patients (PubMed:15456787). Present in the Lewy bodies specifically found in neurons from Parkinson disease patients (PubMed:15456787). Recruited to the cytoplasmic surface of the endoplasmic reticulum via interaction with AMFR/gp78 (PubMed:16168377). Following DNA double-strand breaks, recruited to the sites of damage (PubMed:22120668). Recruited to stalled replication forks via interaction with SPRTN (PubMed:23042605). Recruited to damaged lysosomes decorated with K48-linked ubiquitin chains (PubMed:27753622). Colocalizes with TIA1, ZFAND1 and G3BP1 in cytoplasmic stress granules (SGs) in
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 318-367
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES7491
UniProt entry
P55072
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

VCPvalosin containing protein

View target guide

VCP identifies valosin containing protein in Homo sapiens (Human). This target record connects NCBI Gene 7415, UniProtKB P55072 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
7415
UniProtKB
P55072
ELK Biotechnology
£220.00