Manufactured by:ELK Biotechnology
RUO

CD318 (phospho Tyr707) rabbit pAb

CD318 (phospho Tyr707) rabbit pAb from ELK Biotechnology

SKU:
ABM109260
Product type
Primary Antibody
TargetCDCP1
ApplicationsWB, ELISA
Species / reactivityHuman, Mouse
Expression systemHighly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. Also expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

CD318 (phospho Tyr707) rabbit pAb is a research-use antibody from ELK Biotechnology for studying CDCP1 in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a transmembrane protein which contains three extracellular CUB domains and acts as a substrate for Src family kinases. The protein plays a role in the tyrosine phosphorylation-dependent regulation of cellular events that are involved in tumor invasion and metastasis. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013],

Additional antibody information

Specificity Phospho-CD318 (Y707) Polyclonal Antibody detects endogenous levels of CD318 protein only when phosphorylated at Y707.
Validation evidence captions Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CD318 (Phospho-Tyr707) Antibody | Western blot analysis of lysates from Jurkat cells, using phospho-CD318 (Phospho-Tyr707) antibody.
Key facts & specifications
Research areas
Antibody Research
Also known as
CDCP1; TRASK; CUB domain-containing protein 1; Membrane glycoprotein gp140; Subtractive immunization M plus HEp3-associated 135 kDa protein; SIMA135; Transmembrane and associated with src kinases; CD antigen CD318
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen
Synthesized phospho-peptide around the phosphorylation site of human CD318 (phospho Tyr707)
Observed band
140,80kD
Cellular localization
[Isoform 1]: Cell membrane ; Single-pass membrane protein . Shedding may also lead to a soluble peptide.; [Isoform 3]: Secreted.
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES7202
UniProt entry
Q9H5V8
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

CDCP1CUB domain containing protein 1

View target guide

CDCP1 identifies CUB domain containing protein 1 in Homo sapiens (Human). This target record connects NCBI Gene 64866, UniProtKB Q9H5V8 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
64866
UniProtKB
Q9H5V8
ELK Biotechnology
£220.00