Manufactured by:ELK Biotechnology
RUO

Gα i-3 rabbit pAb

Gα i-3 rabbit pAb from ELK Biotechnology

SKU:
ABM108628
Product type
Primary Antibody
TargetGNAI3
ApplicationsWB, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemLiver,
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Gα i-3 rabbit pAb is a research-use antibody from ELK Biotechnology for studying GNAI3 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling pathways. G proteins are composed of 3 units: alpha, beta and gamma. This gene encodes an alpha subunit and belongs to the G-alpha family. Mutation in this gene, resulting in a gly40-to-arg substitution, is associated with auriculocondylar syndrome, and shown to affect downstream targets in the G protein-coupled endothelin receptor pathway. [provided by RefSeq, Jun 2012],

Additional antibody information

Specificity Gα i-3 Polyclonal Antibody detects endogenous levels of Gα i-3 protein.
Validation evidence captions Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°over night
Key facts & specifications
Research areas
>>Rap1 signaling pathway, >>cGMP-PKG signaling pathway, >>cAMP signaling pathway, >>Chemokine signaling pathway, >>Sphingolipid signaling pathway, >>Adrenergic signaling in cardiomyocytes, >>Axon guidance, >>Apelin signaling pathway, >>Gap junction, >>Platelet activation, >>Leukocyte transendothelial migration, >>Circadian entrainment, >>Retrograde endocannabinoid signaling, >>Glutamatergic synapse, >>Cholinergic synapse, >>Serotonergic synapse, >>GABAergic synapse, >>Dopaminergic synapse, >>Long-term depression, >>Progesterone-mediated oocyte maturation, >>Estrogen signaling pathway, >>Melanogenesis, >>Oxytocin signaling pathway, >>Regulation of lipolysis in adipocytes, >>Renin secretion, >>Relaxin signaling pathway, >>Parathyroid hormone synthesis, secretion and action, >>Cushing syndrome, >>Growth hormone synthesis, >>Gastric acid secretion, >>Parkinson disease, >>Cocaine addiction, >>Morphine addiction, >>Alcoholism, >>Pertussis, >>Chagas disease, >>Toxoplasmosis, >>Human cytomegalovirus infectio
Also known as
GNAI3; Guanine nucleotide-binding protein G(k) subunit alpha; G(i) alpha-3
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human GNAI3. AA range:31-80
Observed band
41kD
Cellular localization
Cytoplasm . Cell membrane ; Lipid-anchor . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody. .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 31-80
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES5567
UniProt entry
P08754
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00