Manufactured by:ELK Biotechnology
RUO

V-ATPase C2 rabbit pAb

V-ATPase C2 rabbit pAb from ELK Biotechnology

SKU:
ABM108556
Product type
Primary Antibody
TargetV-ATPase C2
ApplicationsWB, ELISA
Species / reactivityHuman, Rat, Mouse
Expression systemKidney and placenta.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

V-ATPase C2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying V-ATPase C2 in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A,three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain C subunit isoforms. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity V-ATPase C2 Polyclonal Antibody detects endogenous levels of V-ATPase C2 protein.
Validation evidence captions Western blot analysis of ATP6V1C2 Antibody. The lane on the right is blocked with the ATP6V1C2 peptide. | Western blot analysis of the lysates from HT-29 cells using ATP6V1C2 antibody.
Key facts & specifications
Research areas
>>Oxidative phosphorylation, >>Metabolic pathways, >>Phagosome, >>mTOR signaling pathway, >>Synaptic vesicle cycle, >>Collecting duct acid secretion, >>Vibrio cholerae infection, >>Epithelial cell signaling in Helicobacter pylori infection, >>Human papillomavirus infection, >>Rheumatoid arthritis
Also known as
ATP6V1C2; V-type proton ATPase subunit C 2; V-ATPase subunit C 2; Vacuolar proton pump subunit C 2
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human ATP6V1C2. AA range:121-170
Observed band
48kD
Cellular localization
vacuolar proton-transporting V-type ATPase, V1 domain,lysosomal membrane,cytosol,proton-transporting V-type ATPase, V1 domain,extracellular exosome,
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 121-170
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES5395
UniProt entry
Q8NEY4
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00