Manufactured by:ELK Biotechnology
RUO

DLC-1 rabbit pAb

DLC-1 rabbit pAb from ELK Biotechnology

SKU:
ABM108148
Product type
Primary Antibody
TargetDLC-1
ApplicationsIHC, IF, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemHighest level of expression in the spleen, with rather lower levels in prostate, testis, ovary, small intestine and colon, but none in the thymus.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

DLC-1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying DLC-1 in IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a GTPase-activating protein (GAP) that is a member of the rhoGAP family of proteins which play a role in the regulation of small GTP-binding proteins. GAP family proteins participate in signaling pathways that regulate cell processes involved in cytoskeletal changes. This gene functions as a tumor suppressor gene in a number of common cancers, including prostate, lung, colorectal, and breast cancers. Multiple transcript variants due to alternative promoters and alternative splicing have been found for this gene.[provided by RefSeq, Apr 2010],

Additional antibody information

Specificity DLC-1 Polyclonal Antibody detects endogenous levels of DLC-1 protein.
Validation evidence captions Immunofluorescence analysis of A549 cells, using RHG07 Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma tissue, using RHG07 Antibody. The picture on the right is blocked with the synthesized peptide.
Key facts & specifications
Research areas
Antibody Research
Also known as
DLC1; ARHGAP7; KIAA1723; STARD12; Rho GTPase-activating protein 7; Deleted in liver cancer 1 protein; DLC-1; HP protein; Rho-type GTPase-activating protein 7; START domain-containing protein 12; StARD12; StAR-related lipid transfer protein
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human RHG07. AA range:61-110
Cellular localization
Cytoplasm. Cell junction, focal adhesion. Membrane; Peripheral membrane protein. Colocalizes with EF1A1 at actin-rich regions in the cell periphery.
Assay type
Antibody validation/application
Source organism
Rabbit
Molecular weight
123kD
Concentration
1 mg/ml
Working concentration
Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 61-110
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES4467
UniProt entry
Q96QB1
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00