Manufactured by:ELK Biotechnology
RUO

ALDH2 rabbit pAb

ALDH2 rabbit pAb from ELK Biotechnology

SKU:
ABM107868
Product type
Primary Antibody
TargetALDH2
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemAdipocyte,Brain,Cajal-Retzius cell,Liver,Lymph,Muscle,Small
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

ALDH2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying ALDH2 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform,

Additional antibody information

Specificity ALDH2 Polyclonal Antibody detects endogenous levels of ALDH2 protein.
Validation evidence captions Western Blot analysis of A549 cells using ALDH2 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-lymph-gland, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded human-lymph-gland, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded mouse-muscle, antibody was diluted at 1:100
Key facts & specifications
Research areas
>>Glycolysis / Gluconeogenesis, >>Ascorbate and aldarate metabolism, >>Fatty acid degradation, >>Valine, leucine and isoleucine degradation, >>Lysine degradation, >>Arginine and proline metabolism, >>Histidine metabolism, >>Tryptophan metabolism, >>beta-Alanine metabolism, >>Glycerolipid metabolism, >>Pyruvate metabolism, >>Pantothenate and CoA biosynthesis, >>Metabolic pathways, >>Biosynthesis of cofactors, >>Alcoholic liver disease
Also known as
ALDH2; ALDM; Aldehyde dehydrogenase, mitochondrial; ALDH class 2; ALDH-E2; ALDHI
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from the N-terminal region of human ALDH2. AA range:41-90
Observed band
56kD
Cellular localization
Mitochondrion matrix.
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 41-90
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES4085
UniProt entry
P05091
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

ALDH2aldehyde dehydrogenase 2 family member

View target guide

ALDH2 (aldehyde dehydrogenase 2 family member), also known as mitochondrial aldehyde dehydrogenase, is a mitochondrial matrix enzyme that oxidises acetaldehyde and other aldehydes. Researchers use ALDH2 to study aldehyde metabolism, alcohol metabolism and mitochondrial responses to carbonyl stress.

Biological function: ALDH2 is the mitochondrial aldehyde dehydrogenase isoform with low affinity constant for acetaldehyde and is localised to the mitochondrial matrix. It catalyses a major step in oxidative alcohol metabolism by converting acetaldehyde to acetate.

Organism
Homo sapiens (Human)
NCBI Gene
217
UniProtKB
P05091
ELK Biotechnology
£220.00