Manufactured by:ELK Biotechnology
RUO

GCK rabbit pAb

GCK rabbit pAb from ELK Biotechnology

SKU:
ABM107082
Product type
Primary Antibody
TargetGCK
ApplicationsWB, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemLung,Pancreas,Placenta,
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

GCK rabbit pAb is a research-use antibody from ELK Biotechnology for studying GCK in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). [provided by RefSeq, Apr 2009],

Additional antibody information

Specificity GCK Polyclonal Antibody detects endogenous levels of GCK protein.
Validation evidence captions Western Blot analysis of various cells using GCK Polyclonal Antibody diluted at 1:1000 | Western Blot analysis of NIH-3T3 cells using GCK Polyclonal Antibody diluted at 1:1000 | Western blot analysis of lysate from NIH/3T3 cells, using GCK antibody.
Key facts & specifications
Research areas
>>Glycolysis / Gluconeogenesis, >>Galactose metabolism, >>Starch and sucrose metabolism, >>Amino sugar and nucleotide sugar metabolism, >>Neomycin, kanamycin and gentamicin biosynthesis, >>Metabolic pathways, >>Carbon metabolism, >>Biosynthesis of nucleotide sugars, >>Insulin signaling pathway, >>Insulin secretion, >>Prolactin signaling pathway, >>Glucagon signaling pathway, >>Type II diabetes mellitus, >>Maturity onset diabetes of the young, >>Central carbon metabolism in cancer
Also known as
GCK; Glucokinase; Hexokinase type IV; HK IV; Hexokinase-4; HK4; Hexokinase-D
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human GCK. AA range:43-92
Observed band
55kD
Cellular localization
Cytoplasm . Nucleus . Mitochondrion . Under low glucose concentrations, GCK associates with GCKR and the inactive complex is recruited to the hepatocyte nucleus. .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 43-92
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES2408
UniProt entry
P35557
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

GCKglucokinase

View target guide

GCK identifies glucokinase in Homo sapiens (Human). This target record connects NCBI Gene 2645, UniProtKB P35557 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
2645
UniProtKB
P35557
ELK Biotechnology
£220.00