p38 (phospho Thr180/Y182) rabbit pAb

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Manufactured by:ELK Biotechnology
RUO

p38 (phospho Thr180/Y182) rabbit pAb

p38 (phospho Thr180/Y182) rabbit pAb from ELK Biotechnology

SKU:
ABM104897
Product type
Primary Antibody
Targetp38
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Mouse, Rat, Guineapig
Expression systemBrain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

p38 (phospho Thr180/Y182) rabbit pAb is a research-use antibody from ELK Biotechnology for studying p38 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat, Guineapig. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding d

Additional antibody information

Specificity Phospho-p38 (T180/Y182) Polyclonal Antibody detects endogenous levels of p38 protein only when phosphorylated at T180/Y182.
Key facts & specifications
Research areas
>>Endocrine resistance, >>MAPK signaling pathway, >>Rap1 signaling pathway, >>FoxO signaling pathway, >>Sphingolipid signaling pathway, >>Oocyte meiosis, >>Cellular senescence, >>Adrenergic signaling in cardiomyocytes, >>VEGF signaling pathway, >>Osteoclast differentiation, >>Signaling pathways regulating pluripotency of stem cells, >>Platelet activation, >>Neutrophil extracellular trap formation, >>Toll-like receptor signaling pathway, >>NOD-like receptor signaling pathway, >>RIG-I-like receptor signaling pathway, >>C-type lectin receptor signaling pathway, >>IL-17 signaling pathway, >>Th1 and Th2 cell differentiation, >>Th17 cell differentiation, >>T cell receptor signaling pathway, >>Fc epsilon RI signaling pathway, >>TNF signaling pathway, >>Leukocyte transendothelial migration, >>Thermogenesis, >>Neurotrophin signaling pathway, >>Retrograde endocannabinoid signaling, >>Dopaminergic synapse, >>Inflammatory mediator regulation of TRP channels, >>GnRH signaling pathway, >>Progesterone-mediated oocyte maturation, >
Also known as
MAPK14; CSBP; CSBP1; CSBP2; CSPB1; MXI2; SAPK2A; Mitogen-activated protein kinase 14; MAP kinase 14; MAPK 14; Cytokine suppressive anti-inflammatory drug-binding protein; CSAID-binding protein; CSBP; MAP kinase MXI2; MAX-interacting protein
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
IF/ICC 1:100-500;WB 1:500-2000;Flow Cyt 1:50-200;IHC-p 1:100-500;ELISA 1:5000-20000
Immunogen
The antiserum was produced against synthesized peptide derived from human p38 MAPK around the phosphorylation site of Thr179 and Tyr181. AA range:151-200
Observed band
38kD
Cellular localization
Cytoplasm . Nucleus .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
IF/ICC 1:100-500;WB 1:500-2000;Flow Cyt 1:50-200;IHC-p 1:100-500;ELISA 1:5000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 151-200
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Guineapig
Identifiers & database references
Supplier SKU / catalogue number
ES1431
UniProt entry
Q16539
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00