PI3 Kinase Class III (phospho-Ser249) rabbit pAb

PI3 Kinase Class III (phospho-Ser249) rabbit pAb

PI3 Kinase Class III (phospho-Ser249) rabbit pAb
Manufactured by:ELK Biotechnology
RUO

PI3 Kinase Class III (phospho-Ser249) rabbit pAb

PI3 Kinase Class III (phospho-Ser249) rabbit pAb from ELK Biotechnology

SKU:
ABM104799
Product type
Primary Antibody
TargetPI 3 Kinase Class 3
ApplicationsWB, IHC, ELISA
Species / reactivityHuman, Rat, Mouse
Expression systemUbiquitously expressed, with a highest expression in skeletal muscle.
PurityThe antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

PI3 Kinase Class III (phospho-Ser249) rabbit pAb is a research-use antibody from ELK Biotechnology for studying PI 3 Kinase Class 3 in WB, IHC, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Additional antibody information

Specificity This antibody detects endogenous levels of Human PI3 Kinase Class III (phospho-Ser249)
Validation evidence captions Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). | Western Blot analysis of 1 Jurkat treated with LPS, 2 Jurkat ,using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000
Key facts & specifications
Research areas
>>Inositol phosphate metabolism, >>Metabolic pathways, >>Phosphatidylinositol signaling system, >>Autophagy - other, >>Autophagy - animal, >>Phagosome, >>Apelin signaling pathway, >>Alzheimer disease, >>Amyotrophic lateral sclerosis, >>Huntington disease, >>Spinocerebellar ataxia, >>Pathways of neurodegeneration - multiple diseases, >>Shigellosis, >>Salmonella infection, >>Tuberculosis, >>Kaposi sarcoma-associated herpesvirus infection
Also known as
Phosphatidylinositol 3-kinase catalytic subunit type 3 (PI3-kinase type 3) (PI3K type 3) (PtdIns-3-kinase type 3) (EC 2.7.1.137) (Phosphatidylinositol 3-kinase p100 subunit) (Phosphoinositide-3-kinase class 3) (hVps34)
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Immunogen
Synthesized phosho peptide around human PI3 Kinase Class III (Ser249)
Observed band
100kD
Cellular localization
Midbody . Late endosome . Cytoplasmic vesicle, autophagosome . As component of the PI3K complex I localized to pre-autophagosome structures. As component of the PI3K complex II localized predominantly to endosomes (PubMed:14617358). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme (By similarity). .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES14121
UniProt entry
Q8NEB9
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00