Manufactured by:ELK Biotechnology
RUO

Stat6 (phospho Thr645) rabbit pAb

Stat6 (phospho Thr645) rabbit pAb from ELK Biotechnology

SKU:
ABM104777
Product type
Primary Antibody
TargetStat6
ApplicationsWB, IHC, IF, ELISA, IP
Species / reactivityHuman, Mouse, Rat
Expression systemUterus,
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Stat6 (phospho Thr645) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Stat6 in WB, IHC, IF, ELISA, IP workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010],

Additional antibody information

Specificity Phospho-Stat6 (T645) Polyclonal Antibody detects endogenous levels of Stat6 protein only when phosphorylated at T645.
Validation evidence captions Western Blot analysis of various cells using Phospho-Stat6 (T645) Polyclonal Antibody | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using STAT6 (Phospho-Thr645) Antibody. The picture on the right is blocked with the phospho peptide.
Key facts & specifications
Research areas
>>Necroptosis, >>JAK-STAT signaling pathway, >>Th1 and Th2 cell differentiation, >>Th17 cell differentiation, >>Hepatitis B, >>Pathways in cancer, >>Inflammatory bowel disease
Also known as
STAT6; Signal transducer and activator of transcription 6; IL-4 Stat
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human STAT6 around the phosphorylation site of Thr645. AA range:612-661
Cellular localization
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
Assay type
Antibody validation/application
Source organism
Rabbit
Molecular weight
94kD
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 612-661
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES1408
UniProt entry
P42226
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

STAT6signal transducer and activator of transcription 6

View target guide

STAT6 identifies signal transducer and activator of transcription 6 in Homo sapiens (Human). This target record connects NCBI Gene 6778, UniProtKB P42226 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
6778
UniProtKB
P42226
ELK Biotechnology
£220.00