AP-1/Jun D (phospho Ser73/100) rabbit pAb

AP-1/Jun D (phospho Ser73/100) rabbit pAb

AP-1/Jun D (phospho Ser73/100) rabbit pAb
Manufactured by:ELK Biotechnology
RUO

AP-1/Jun D (phospho Ser73/100) rabbit pAb

AP-1/Jun D (phospho Ser73/100) rabbit pAb from ELK Biotechnology

SKU:
ABM104070
Product type
Primary Antibody
Targetc-JUN
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemExpressed in the developing and adult prostate and prostate cancer cells.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

AP-1/Jun D (phospho Ser73/100) rabbit pAb is a research-use antibody from ELK Biotechnology for studying c-JUN in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity Phospho-AP-1 (S73) Polyclonal Antibody detects endogenous levels of AP-1 protein only when phosphorylated at S73.
Validation evidence captions Western Blot analysis of various cells using Phospho-AP-1/Jun D (S73/100) Polyclonal Antibody diluted at 1:1000 | Western Blot analysis of HuvEc cells using Phospho-AP-1/Jun D (S73/100) Polyclonal Antibody diluted at 1:1000 | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using c-Jun (Phospho-Ser73) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from HUVEC cells treated with TNF 20ng/ml 5' and K562 cells treated with TNF 20ng/ml 5', using c-Jun (Phospho-Ser73) Antibody. The lane on the right is blocked with the phospho peptide.
Key facts & specifications
Research areas
>>Endocrine resistance, >>MAPK signaling pathway, >>ErbB signaling pathway, >>cAMP signaling pathway, >>Mitophagy - animal, >>Apoptosis, >>Wnt signaling pathway, >>Osteoclast differentiation, >>Focal adhesion, >>Tight junction, >>Toll-like receptor signaling pathway, >>NOD-like receptor signaling pathway, >>C-type lectin receptor signaling pathway, >>IL-17 signaling pathway, >>Th1 and Th2 cell differentiation, >>Th17 cell differentiation, >>T cell receptor signaling pathway, >>B cell receptor signaling pathway, >>TNF signaling pathway, >>Neurotrophin signaling pathway, >>GnRH signaling pathway, >>Estrogen signaling pathway, >>Oxytocin signaling pathway, >>Relaxin signaling pathway, >>Non-alcoholic fatty liver disease, >>AGE-RAGE signaling pathway in diabetic complications, >>Cocaine addiction, >>Amphetamine addiction, >>Epithelial cell signaling in Helicobacter pylori infection, >>Pathogenic Escherichia coli infection, >>Shigellosis, >>Salmonella infection, >>Pertussis, >>Yersinia infection, >>Leishmaniasis, >>Chagas di
Also known as
JUN; Transcription factor AP-1; Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39; JUND; Transcription factor jun-D
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human c-Jun around the phosphorylation site of Ser73. AA range:40-89
Observed band
39-42kD
Cellular localization
Nucleus.
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 40-89
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES1261
UniProt entry
P05412/P17535
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00