Manufactured by:ELK Biotechnology
RUO

TRIM27 rabbit pAb

TRIM27 rabbit pAb from ELK Biotechnology

SKU:
ABM104046
Product type
Primary Antibody
TargetTRIM27
ApplicationsWB
Species / reactivityHuman, Mouse
Expression systemExpressed in testis namely within the seminiferous tubules.
PurityThe antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

TRIM27 rabbit pAb is a research-use antibody from ELK Biotechnology for studying TRIM27 in WB workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity This antibody detects endogenous levels of TRIM27 at Human/Mouse
Validation evidence captions Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night
Key facts & specifications
Research areas
Antibody Research
Also known as
Zinc finger protein RFP (EC 6.3.2.-) (RING finger protein 76) (Ret finger protein) (Tripartite motif-containing protein 27)
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000
Immunogen
Synthesized peptide derived from human TRIM27 AA range: 34-84
Observed band
60kD
Cellular localization
Nucleus . Cytoplasm . Nucleus, PML body . Early endosome . Nuclear or cytoplasmic depending on the cell type (By similarity). Colocalized with PML and EIF3S6 in nuclear bodies. Recruited to retromer-containing endosomes via interaction with MAGEL2 (PubMed:23452853). .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 34-84
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES12563
UniProt entry
P14373
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.10% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00