Manufactured by:ELK Biotechnology
RUO

BMAL1 (Acetyl Lys538) rabbit pAb

BMAL1 (Acetyl Lys538) rabbit pAb from ELK Biotechnology

SKU:
ABM103132
Product type
Primary Antibody
TargetBMAL1
ApplicationsWB, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemHair follicles (at protein level). Highly expressed in the adult brain, skeletal muscle and heart.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

BMAL1 (Acetyl Lys538) rabbit pAb is a research-use antibody from ELK Biotechnology for studying BMAL1 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014],

Additional antibody information

Specificity Acetyl-BMAL1 (K538) Polyclonal Antibody detects endogenous levels of BMAL1 protein only when acetylated at K538.
Validation evidence captions Western Blot analysis of 293 cells using Acetyl-BMAL1 (K538) Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western blot analysis of lysate from 293 cells, using BMAL1 (Acetyl-Lys538) Antibody.
Key facts & specifications
Research areas
>>Circadian rhythm, >>Dopaminergic synapse
Also known as
ARNTL; BHLHE5; BMAL1; MOP3; PASD3; Aryl hydrocarbon receptor nuclear translocator-like protein 1; Basic-helix-loop-helix-PAS protein MOP3Brain and muscle ARNT-like 1; Class E basic helix-loop-helix protein 5; bHLHe5; Member of PAS protein 3; PAS domain-containing protein 3; bHLH-PAS protein JAP3
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized Acetyl-peptide derived from human BMAL1 around the Acetylation site of Lys538. AA range:501-550
Observed band
70kD
Cellular localization
Nucleus . Cytoplasm . Nucleus, PML body . Shuttles between the nucleus and the cytoplasm and this nucleocytoplasmic shuttling is essential for the nuclear accumulation of CLOCK, target gene transcription and the degradation of the CLOCK-ARNTL/BMAL1 heterodimer. The sumoylated form localizes in the PML body. Sequestered to the cytoplasm in the presence of ID2. .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 501-550
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES1116
UniProt entry
O00327
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

BMAL1basic helix-loop-helix ARNT like 1

View target guide

Basic helix-loop-helix ARNT like 1 (BMAL1; ARNTL) is a core bHLH-PAS circadian transcription factor. BMAL1 heterodimerizes with CLOCK or NPAS2 to drive E-box-dependent transcription that helps generate daily rhythms in gene expression, metabolism and physiology.

Biological function: BMAL1 forms transcriptionally active heterodimers with CLOCK or NPAS2 and binds E-box regulatory elements in circadian target genes. Its activity drives molecular-clock transcriptional programs and supports feedback loops that generate approximately 24-hour rhythms in gene expression.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
406
UniProtKB
O00327
ELK Biotechnology
£220.00