Manufactured by:ELK Biotechnology
RUO

Cleaved-COL3A1 (G1221) rabbit pAb

Cleaved-COL3A1 (G1221) rabbit pAb from ELK Biotechnology

SKU:
ABM102662
Product type
Primary Antibody
TargetCollagen III
ApplicationsELISA
Species / reactivityHuman, Rat, Mouse
Expression systemColon carcinoma,Liver,Placenta,Skin fibroblast,
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Cleaved-COL3A1 (G1221) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Collagen III in ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

collagen type III alpha 1 chain(COL3A1) Homo sapiens This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008],

Additional antibody information

Specificity Cleaved-COL3A1 (G1221) Polyclonal Antibody detects endogenous levels of fragment of activated COL3A1 protein resulting from cleavage adjacent to G1221.
Validation evidence captions Western Blot analysis of various cells using Cleaved-COL3A1 (G1221) Polyclonal Antibody | Western blot analysis of lysates from A549 cells, treated with etoposide 25uM 24h, using Collagen III alpha1 (Cleaved-Gly1221) Antibody. The lane on the right is blocked with the synthesized peptide.
Key facts & specifications
Research areas
>>Platelet activation, >>Relaxin signaling pathway, >>AGE-RAGE signaling pathway in diabetic complications, >>Protein digestion and absorption, >>Amoebiasis, >>Diabetic cardiomyopathy
Also known as
COL3A1; Collagen alpha-1(III) chain
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
ELISA: 1/10000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human Collagen III alpha1. AA range:1172-1221
Observed band
95kD
Cellular localization
Secreted, extracellular space, extracellular matrix .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 1172-1221
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES1035
UniProt entry
P02461
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00