NFkB p65 Mouse mAb from ELK Biotechnology
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NFkB p65 Mouse mAb is a research-use antibody from ELK Biotechnology for studying NFkB p65 in WB, IHC, IF, IP workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
NFkB p65 is ubiquitinated leading to its proteosomal degradation, which is required for termination of the NFkB response. Phosphorylation of NFkB p65 on S536 stimulates acetylation of K310 by CBP, enhancing transcriptional activity. NFkB p65 is also acetylated at K122, enhancing DNA binding and impairing the interaction with NFKBIA. The protein is deacetylated by HDAC3. Invasion of a host by a pathogen is frequently associated with the activation of NF-kB, which coordinates various aspects of immune function required for resistance to infection.
| Validation evidence captions | Western blot analysis of extracts from HeLa (Lane 1),MCF-7 (Lane 2), Mouse Liver (Lane 3), Rat Kidney (Lane 4), using NFκB p65 diluted at 1:1,500. | 1、Input: Hela Cell Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1111 1:2,000 Secondary antibody: Goat anti-Mouse IgG, Light chain specific(S003), 1:5,000 | IF analysis of Hela with EM1111(Left) and DAPI (Right) diluted at 1:100. | Immunohistochemical analysis of paraffin-embedded Rat Brain Tissue using NFkB p65 （EM1111）Mouse mAbdiluted at 1:500. |
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Fully tested in-house by ABMIUM. Highest confidence. Non-conformities fully supported under the ABMIUM Product Promise.
Quality verified. Independent collaborator or expected-performance data available. Product meets ABMIUM quality standards.
Expected to work based on structural and biochemical data. Not yet directly tested by ABMIUM or a collaborator. No non-conformity support for this combination.
Not recommended for this application or species/sample combination. This may indicate either evidence of unsuitability or that the combination has not been tested and therefore cannot currently be recommended.
Evidence status by application and model.
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.
ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.
Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.
Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.
