Manufactured by:ELK Biotechnology
RUO

Sheep vWF(Von Willebrand Factor) ELISA Kit

Sheep vWF(Von Willebrand Factor) ELISA Kit from ELK Biotechnology

SKU:
ABM50927
Product type
ELISA Kits
TargetvWF
ApplicationsELISA
Species / reactivitySheep
Sample typePlasma

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Sheep vWF(Von Willebrand Factor) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring vWF in Plasma samples from Sheep. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep vWF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep vWF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep vWF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep vWF in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include F8VWF; VWD; von Willebrand antigen 2.

Key facts & specifications
Research areas
Signal transduction, Hematology
Also known as
F8VWF; VWD; von Willebrand antigen 2
Assay type
Sandwich
Detection method
Colorimetric (TMB/HRP)
Sensitivity
0.049 µg/mL
Detection range
0.16-10 µg/mL
Plate format
96-well pre-coated
Form / buffer
Pre-coated microplate kit
Quantity per pack
48T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Sample type validation

Sample compatibility evidence using the same validation key.

Sample typePlasma
Sample compatibility
Identifiers & database references
Supplier SKU / catalogue number
ELK10102
Storage, stability & shipping
Storage conditions
Store according to the conditions stated in the product manual.
Long-term storage
Store according to the conditions stated in the product manual.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

VWFvon Willebrand factor

View target guide

Von Willebrand factor (VWF; also F8VWF) is a multimeric plasma glycoprotein central to primary hemostasis. This ABMIUM target record links human VWF (NCBI Gene 7450; UniProt P04275) to catalogue-backed research products.

Biological function: Von Willebrand factor (VWF) supports platelet adhesion at sites of vascular injury and participates in transport of proteins in blood. Its multimeric structure is central to normal hemostatic function.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
7450
UniProtKB
P04275
ELK Biotechnology
£366.00