Manufactured by:ELK Biotechnology
RUO

cPLA2-γ rabbit pAb

cPLA2-γ rabbit pAb from ELK Biotechnology

SKU:
ABM109483
Product type
Primary Antibody
TargetcPLA2-γ
ApplicationsWB, ELISA
Species / reactivityHuman, Rat, Mouse
Expression systemHighly expressed in heart and skeletal muscle.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

cPLA2-γ rabbit pAb is a research-use antibody from ELK Biotechnology for studying cPLA2-γ in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a protein which is a member of the phospholipase A2 enzyme family which hydrolyzes glycerophospholipids to produce free fatty acids and lysophospholipids, both of which serve as precursors in the production of signaling molecules. The encoded protein has been shown to be a calcium-independent and membrane bound enzyme. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009],

Additional antibody information

Specificity cPLA2-γ Polyclonal Antibody detects endogenous levels of cPLA2-γ protein.
Validation evidence captions Western blot analysis of lysates from Jurkat cells, using PLA2G4C Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HeLa cells using PLA2G4C antibody.
Key facts & specifications
Research areas
>>Glycerophospholipid metabolism, >>Ether lipid metabolism, >>Arachidonic acid metabolism, >>Linoleic acid metabolism, >>alpha-Linolenic acid metabolism, >>Metabolic pathways, >>MAPK signaling pathway, >>Ras signaling pathway, >>Phospholipase D signaling pathway, >>Necroptosis, >>Vascular smooth muscle contraction, >>VEGF signaling pathway, >>Platelet activation, >>Fc epsilon RI signaling pathway, >>Fc gamma R-mediated phagocytosis, >>Glutamatergic synapse, >>Serotonergic synapse, >>Long-term depression, >>Inflammatory mediator regulation of TRP channels, >>GnRH signaling pathway, >>Ovarian steroidogenesis, >>Oxytocin signaling pathway, >>Choline metabolism in cancer
Also known as
PLA2G4C; Cytosolic phospholipase A2 gamma; cPLA2-gamma; Phospholipase A2 group IVC
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human PLA2G4C. AA range:301-350
Observed band
70kD
Cellular localization
Cell membrane ; Lipid-anchor . Endoplasmic reticulum membrane ; Lipid-anchor. Mitochondrion membrane ; Lipid-anchor. Lipid droplet . Translocates from endoplasmic reticulum to lipid droplets in response to oleate. .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 301-350
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES7831
UniProt entry
Q9UP65
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00